Long non-coding RNA DLX6 antisense RNA 1 (DLX6-AS1) lists a critical position in thyroid carcinoma (TC) development. However, the overall comprehension about DLX6-AS1, microRNA (miR)-193b-3p and homeobox A1 (HOXA1) in TC is not thoroughly enough. Concerning to this, this work is pivoted on DLX6-AS1/miR-193b-3p/HOXA1 axis in TC cell growth and autophagy. TC tissues and adjacent normal thyroid tissues were collected, in which expression of DLX6-AS1, miR-193b-3p and HOXA1 was tested, together with their interactions. TC cells were transfected with DLX6-AS1/miR-193b-3p-related oligonucleotides or plasmids to test cell growth and autophagy. Tumorigenesis in nude mice was observed. DLX6-AS1 and HOXA1 were up-regulated, and miR-193b-3p was down-regulated in TC. Depleted DLX6-AS1 or restored miR-193b-3p disturbed cell growth and promoted autophagy. DLX6-AS1 targeted miR-193b-3p and positively regulated HOXA1. miR-193b-3p inhibition mitigated the impaired tumorigenesis induced by down-regulated DLX6-AS1. Tumorigenesis in nude mice was consistent with that in cells. It is clear that DLX6-AS1 depletion hinders TC cell growth and promotes autophagy via up-regulating miR-193b-3p and down-regulating HOXA1.
第一作者机构：[1]Capital Med Univ, Beijing Tongren Hosp, Dept Otorhinolaryngol Head & Neck Surg, 1 Dongjiao Minxiang, Beijing 100730, Peoples R China[2]Minist Educ, Key Lab Otorhinolaryngol Head & Neck Surg, Beijing, Peoples R China
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Tongren Hosp, Dept Otorhinolaryngol Head & Neck Surg, 1 Dongjiao Minxiang, Beijing 100730, Peoples R China[2]Minist Educ, Key Lab Otorhinolaryngol Head & Neck Surg, Beijing, Peoples R China[*1]Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No. 1 Dongjiao Minxiang, Dongcheng District, Beijing 100730, China and Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Beijing, China.
推荐引用方式(GB/T 7714)：
Feng Ling,Wang Ru,Wang Yifan,et al.Silencing long non-coding RNA DLX6-AS1 or restoring microRNA-193b-3p enhances thyroid carcinoma cell autophagy and apoptosis via depressing HOXA1[J].JOURNAL OF CELLULAR AND MOLECULAR MEDICINE.2021,25(19):9319-9330.doi:10.1111/jcmm.16868.
APA：
Feng, Ling,Wang, Ru,Wang, Yifan,Shen, Xixi,Shi, Qian...&Fang, Jugao.(2021).Silencing long non-coding RNA DLX6-AS1 or restoring microRNA-193b-3p enhances thyroid carcinoma cell autophagy and apoptosis via depressing HOXA1.JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,25,(19)
MLA：
Feng, Ling,et al."Silencing long non-coding RNA DLX6-AS1 or restoring microRNA-193b-3p enhances thyroid carcinoma cell autophagy and apoptosis via depressing HOXA1".JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 25..19(2021):9319-9330
