机构：[1]Department of Anesthesiology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China[2]Department of Urology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China[3]Department of Urology, Zhongshan Hospital Qingpu Branch, Fudan University, Shanghai, China
To elucidate the correlation between microRNA-1266 (miR-1266) and prostate cancer (PCa) progression, and to investigate the possible underlying mechanism.The expression level of miR-1266 and protein arginine methyltransferase 5 (PRMT5) in PCa tissues and cell lines was first detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). After up-regulating or down-regulating miR-1266 expression in cells, cell proliferation, migration and invasion abilities were detected. Possible target genes of miR-1266 were predicted and validated by bioinformatics analysis and dual-luciferase reporter gene assay, respectively. Finally, abnormal expression of PRMT5 was ascertained after transfection.MiR-1266 was lowly expressed in PCa tissues and cell lines, whereas PRMT5 exhibited the opposite results. Up-regulated expression of miR-1266 significantly inhibited the proliferation, migration and invasion abilities of PC-3 cells. However, the growth and migration of DU145 cells with low miR-1266 expression were significantly accelerated. Meanwhile, the number of invading cells was significantly increased. PRMT5 was verified as a potential target gene of miR-1266. Furthermore, results found that miR-1266 was negatively correlated with PRMT5. In addition, the expression of PRMT5 was remarkably decreased after miR-1266 overexpression, which could be restored after knockdown of miR-1266.MiR-1266 inhibits the growth and metastasis of PCa by targeting PRMT5. We may provide a potential and prospective therapeutic target for PCa.
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外文
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中科院(CAS)分区：
出版当年[2018]版：
大类|4 区医学
小类|4 区药学
最新[2025]版：
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第一作者：
第一作者机构：[1]Department of Anesthesiology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
通讯作者：
推荐引用方式(GB/T 7714)：
Sun C-M,Zhang G-M,Qian H-N,et al.MiR-1266 suppresses the growth and metastasis of prostate cancer via targeting PRMT5.[J].European Review For Medical And Pharmacological Sciences.2019,23(15):6436-6444.doi:10.26355/eurrev_201908_18525.
APA：
Sun C-M,Zhang G-M,Qian H-N,Cheng S-J,Wang M...&Li D.(2019).MiR-1266 suppresses the growth and metastasis of prostate cancer via targeting PRMT5..European Review For Medical And Pharmacological Sciences,23,(15)
MLA：
Sun C-M,et al."MiR-1266 suppresses the growth and metastasis of prostate cancer via targeting PRMT5.".European Review For Medical And Pharmacological Sciences 23..15(2019):6436-6444
