资源类型：
期刊
WOS体系：
Article
Pubmed体系：
Journal Article
收录情况：
◇ SCIE
文章类型：
论著
机构：
[1]Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
[2]State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China
[3]State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing, China
江苏省人民医院
ISSN：
0006-291X
关键词：
Transfer RNA fragment
Obesity
Forskolin
Exosomes
Brown adipocyte
Lipolysis
摘要：
Human obesity is related with intrinsic impairments of adipocyte lipolysis and ectopic lipid accumulation. Small regulatory RNAs, such as tRNA-derived fragments (tRFs) and tRNA halves (tiRNAs), are enriched in exosomes and play a crucial role in lipid metabolism. To determine certain tRFs for lipolysis, brown adipocytes were treated with forskolin. Using tRFs sequencing, 207 different expressed exosomal tRFs were determined. In forskolin samples, 145 downregulated and 62 upregulated tRFs were identified. Further, qRT-PCR validated that three notably upregulated tRFs (tRF-Gly-GCC-007, tRF-Gly-GCC-008, and tRF-Gly-GCC-009) were in accordance with the sequencing result. Target genes of tRFs were involved in positive regulation of protein phosphorylation and cell adhesion process by significantly downregulating UCHL1 expression, which might participate in lipolysis. This study might provide therapeutic targets and potential diagnostic biomarkers for obesity treatment.Copyright © 2023 Elsevier Inc. All rights reserved.
基金：
This study was supported by grants from the National Natural
Science Foundation of China (Grant No. 82001508 to Siliang Xu,
82170869 to Xirong Guo, 82200951 to Zhou Peng) and Large-scale
Scientific Instruments Opening and Sharing Mechanism Project of
Nanjing Medical University (Grant No. ZC2021DY11 to Jinyang Cai).
被引次数：
2
WOS：
WOS:001030086700001
PubmedID：
37352601
中科院(CAS)分区：
出版当年[2022]版：
大类
|
4 区
生物学
小类
|
4 区
生化与分子生物学
4 区
生物物理
最新[2025]版：
大类
|
4 区
生物学
小类
|
4 区
生化与分子生物学
4 区
生物物理
JCR分区：
出版当年[2021]版：
Q3
BIOCHEMISTRY & MOLECULAR BIOLOGY
Q3
BIOPHYSICS
最新[2024]版：
Q3
BIOPHYSICS
Q4
BIOCHEMISTRY & MOLECULAR BIOLOGY
影响因子：
2.2
最新[2024版]
2.5
最新五年平均
3.322
出版当年[2021版]
3.498
出版当年五年平均
3.575
出版前一年[2020版]
3.1
出版后一年[2022版]
第一作者：
Peng Zhou
第一作者机构：
[1]Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
共同第一作者：
Cai Jingyang
通讯作者：
Guo Xirong;Xu Siliang
推荐引用方式(GB/T 7714)：
Peng Zhou,Cai Jingyang,Guo Xirong,et al.Brown adipocyte activation mediates lipid metabolism through exosomal tRNA-derived fragments[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2023,672:128-136.doi:10.1016/j.bbrc.2023.06.038.
APA：
Peng Zhou,Cai Jingyang,Guo Xirong&Xu Siliang.(2023).Brown adipocyte activation mediates lipid metabolism through exosomal tRNA-derived fragments.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,672,
MLA：
Peng Zhou,et al."Brown adipocyte activation mediates lipid metabolism through exosomal tRNA-derived fragments".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 672.(2023):128-136
