机构：[1]Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China[2]Capital Med Univ, Beijing Tongren Hosp, Dept Stomatol, Beijing, Peoples R China临床科室口腔科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[3]Naval Med Univ, Dept Dermatol, Affiliated Hosp 2, Shanghai, Peoples R China[4]Fudan Univ, Huashan Hosp, Shanghai Inst Infect Dis & Biosecur, Sch Life Sci,Dept Infect Dis, Shanghai, Peoples R China[5]Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai, Peoples R China[6]Chongqing Med Univ, Coll Stomatol, Sch Life Sci, State Key Lab Genet Engn, Chongqing 401147, Peoples R China
Candida albicans is a normal resident of the human oral cavity. It is also the most common fungal pathogen, causing various oral diseases, particularly in immunocompromised individuals. Chlorhexidine digluconate (CHG) is a broad-spectrum antimicrobial agent widely used in dental practice and has been recommended to treat oral candidiasis. However, its action mechanism against the fungal pathogen C. albicans remains poorly understood. The aim of the present study was to investigate the effect of CHG at sub-lethal concentrations against C. albicans. CHG inhibited the growth of C. albicans in a dose- and time-dependent manner. Cells treated with CHG exhibited altered membrane permeability, reduced metabolic activity, and enhanced metal ion and reactive oxygen species (ROS) accumulation. Copper-sensing transcription factor Mac1, iron-sensing transcription factors Sfu1 and Sef2, and copper transporter Ctr1 regulated intracellular metal ion and ROS homeostasis in response to CHG. Deletion of MAC1, SFU1, or SEF2 increased intracellular ROS production and cell susceptibility to CHG. This study revealed a novel mechanism by which CHG induced apoptosis of C. albicans cells through the disruption of metal ion and ROS homeostasis, which may help to identify new targets for fungal infections.
基金：
This work was supported by the National Key Research and Development Program of China [Grant Number 2021YFC2300400 to LT], National Natural Science Foundation of China [Grant Number 32170194 to LT], Natural Science Foundation of Shanghai award [20ZR1405900 to LT], Top Talent Distinguished Professor from Chongqing Medical University [No. (2021)215 to DY], and Program for Youth Innovation in Future Medicine from Chongqing Medical University [No. W0060 to DY].
第一作者机构：[1]Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China[2]Capital Med Univ, Beijing Tongren Hosp, Dept Stomatol, Beijing, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China[4]Fudan Univ, Huashan Hosp, Shanghai Inst Infect Dis & Biosecur, Sch Life Sci,Dept Infect Dis, Shanghai, Peoples R China[5]Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai, Peoples R China[6]Chongqing Med Univ, Coll Stomatol, Sch Life Sci, State Key Lab Genet Engn, Chongqing 401147, Peoples R China
推荐引用方式(GB/T 7714)：
Jiang Qian,Deng Yuchen,Li Shuaihu,et al.Sub-lethal concentrations of chlorhexidine inhibit Candida albicans growth by disrupting ROS and metal ion homeostasis[J].JOURNAL OF ORAL MICROBIOLOGY.2023,15(1):doi:10.1080/20002297.2023.2278937.
APA：
Jiang, Qian,Deng, Yuchen,Li, Shuaihu,Yang, Deqin&Tao, Li.(2023).Sub-lethal concentrations of chlorhexidine inhibit Candida albicans growth by disrupting ROS and metal ion homeostasis.JOURNAL OF ORAL MICROBIOLOGY,15,(1)
MLA：
Jiang, Qian,et al."Sub-lethal concentrations of chlorhexidine inhibit Candida albicans growth by disrupting ROS and metal ion homeostasis".JOURNAL OF ORAL MICROBIOLOGY 15..1(2023)
