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Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen

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收录情况： ◇ ESCI

作者：
Gao Zili[1,3] * ; Ding Shenglong[2] * ; Fan Tingting[1,3] * ; Huang Wenhui[1,3] * ; Zhao Xiyuan[1,3] ; Liu Xin[1,3,4] ; Liu Wenli[1,3] ; Zhang Mingzhu[2] ; Gu Qi[1,3] ;
机构： [1]Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China [2]Department of Foot and Ankle Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China. [3]Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. China. [4]Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, Jilin University, Changchun 130061, P.R. China.
出处：
DOI： 10.1016/j.xpro.2024.102994
ISSN：

摘要：
Here, we present a protocol for 3D printing heart tissues using thiol-norbornene photoclick collagen (NorCol). We describe steps for synthesizing NorCol, preparing bioink and the support bath, and cell-laden printing. We then detail procedures for the loading of C2C12 cells into NorCol, ensuring structural integrity and cell viability after printing. This protocol is adaptable to various cell lines and allows for the printing of diverse complex structures, which can be used in drug screening and disease modeling.Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.

基金：
This research was supported by the National Natural Science Foundation of China (T2222029, U21A20396, 62127811, U23A20453, and 82004050), the National Key Research and Development Program of China (2022YFA1104701), the CAS Project for Young Scientists in Basic Research (YSBR- 012), the CAS Pioneer Hundred Talents Program (Y829F11102), the CAS Engineering Laboratory for Intelligent Organ Manufacturing (KFJ-PTXM-039), the Incubation Foundation of Beijing Institute for Stem Cell and RegenerativeMedicine (2022FH107), the Beijing Tongren Hospital Top Talent Project (2024-YJJ-BJL-016), and the China Postdoctoral Science Foundation (2022M710903).
语种：
外文
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中科院(CAS)分区：
出版当年[2023]版：
无
最新[2025]版：
大类 | 4 区 生物学
小类 | 4 区 生化研究方法
JCR分区：
出版当年[2022]版：
无
最新[2024]版：
Q4 BIOCHEMICAL RESEARCH METHODS

影响因子： 最新[2024版] 最新五年平均 出版当年[2022版] 出版当年五年平均 出版前一年[2021版] 出版后一年[2023版]

第一作者：
第一作者机构： [1]Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China [3]Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. China.
共同第一作者：
通讯作者：
通讯机构： [1]Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China [3]Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. China.
推荐引用方式(GB/T 7714)：
Gao Zili,Ding Shenglong,Fan Tingting,et al.Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen[J].STAR PROTOCOLS.2024,5(2):102994.doi:10.1016/j.xpro.2024.102994.
APA：
Gao Zili,Ding Shenglong,Fan Tingting,Huang Wenhui,Zhao Xiyuan...&Gu Qi.(2024).Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen.STAR PROTOCOLS,5,(2)
MLA：
Gao Zili,et al."Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen".STAR PROTOCOLS 5..2(2024):102994

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