Objective: To evaluate the accuracy of airborne pollen concentrations monitored by a novel volumetric suction pollen monitor. Methods: During the peak period of autumn pollen concentration in Beijing from September 1st to 27th, the pollen concentration was collected using gravitational and volumetric pollen monitor, and the correlation of pollen concentration between two kind of pollen monitor was analyzed. The effects of daily mean temperature, relative humidity and wind speed on the real-time pollen concentration of the volumetric suction pollen monitor were also analyzed. SPSS 25.0 software was used for statistical analysis. Results: There was significant correlation between the pollen concentration obtained by the two collectors (rs value was 0.85 and 0.86, respectively, both P<0.001). Using a volumetric suction monitor, the pollen concentration in the horizontal 3 lines of the pollen sample (12.5%) under a 200×microscope was significantly correlated with the pollen concentration in the whole sample (rs=0.97, P<0.001). The real-time pollen concentration collected by the volumetric suction monitor within 24 hours was greatly affected by the daily average temperature (all P<0.05), and the pollen concentration increased with the daytime temperature, which was not affected by the relative humidity and the average wind speed (all P>0.05). Conclusion: The airborne pollen concentration monitored by new volumetric pollen monitor has high accuracy and can accurately achieve real-time.