Plasma concentrations of nicotine and its active metabolite cotinine are highly correlated with its biological effects. A UHPLC-MS/MS method was developed, validated and applied for nicotine and cotinine analysis in mice plasma. Chromatographic separation was achieved on a BEH HILIC column using acetonitrile (0.1% formic acid) and 10 mm ammonium formate as mobile phase. The gradient elution was performed at 0.4 mL/min with a run time of 3.6 min. The quantitative ion transition was m/z 163.1 > 130.0 for nicotine, m/z 177.1 > 80.0 for cotinine and m/z 167.1 > 134.0 for nicotine-D-4 (internal standard, IS). For both nicotine and cotinine, the calibration range was 5-500 ng/mL with 5 ng/mL as the lower limit of quantitation, and the intra- and inter-day bias and imprecision were -4.61-12.00% and <11.12%. The IS normalized recovery was 90.62-98.95% for nicotine and 89.18-101.53% for cotinine, and the IS normalized matrix factor was 106.00-116.44% for nicotine and 100.34-109.85% for cotinine. Both nicotine and cotinine were stable under conventional storage conditions. The validated method has been applied to a pharmacokinetic study in mice to calculate the pharmacokinetic parameters for both analytes.
基金：
Beijing Municipal Administration of Hospitals [ZYLX201827]; Beijing Natural Science FoundationBeijing Natural Science Foundation [7172087]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|4 区生物
小类|4 区生化研究方法4 区生化与分子生物学4 区分析化学4 区药学
最新[2025]版：
大类|4 区医学
小类|4 区生化研究方法4 区生化与分子生物学4 区分析化学4 区药学
JCR分区：
出版当年[2017]版：
Q3CHEMISTRY, ANALYTICALQ3BIOCHEMICAL RESEARCH METHODSQ4BIOCHEMISTRY & MOLECULAR BIOLOGYQ4PHARMACOLOGY & PHARMACY
最新[2024]版：
Q3CHEMISTRY, ANALYTICALQ3PHARMACOLOGY & PHARMACYQ4BIOCHEMICAL RESEARCH METHODSQ4BIOCHEMISTRY & MOLECULAR BIOLOGY
第一作者机构：[1]Capital Med Univ, Sch Stomatol, Lab Tissue Regenerat & Immunol, 4 Tiantanxili, Beijing 100050, Peoples R China[2]Capital Med Univ, Sch Stomatol, Dept Periodont, Beijing Key Lab Tooth Regenerat & Funct Reconstru, 4 Tiantanxili, Beijing 100050, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Capital Med Univ, Sch Stomatol, Lab Tissue Regenerat & Immunol, 4 Tiantanxili, Beijing 100050, Peoples R China[2]Capital Med Univ, Sch Stomatol, Dept Periodont, Beijing Key Lab Tooth Regenerat & Funct Reconstru, 4 Tiantanxili, Beijing 100050, Peoples R China[3]Capital Med Univ, Beijing Tiantan Hosp, Dept Pharm, 119 Nansihuan West Rd, Beijing 100070, Peoples R China[4]Capital Med Univ, Coll Pharmaceut Sci, Dept Clin Pharmacol, Beijing, Peoples R China[*1]Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, 119 Nansihuan West Road, Fengtai District, Beijing 100070, P. R. China.[*2]Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, 4 Tiantanxili, Beijing 100050, P. R. China.
推荐引用方式(GB/T 7714)：
Liu Yang,Zhang Dongjie,Du Juan,et al.Simultaneous determination of plasma nicotine and cotinine by UHPLC-MS/MS in C57BL/6 mice and its application in a pharmacokinetic study[J].BIOMEDICAL CHROMATOGRAPHY.2019,33(10):doi:10.1002/bmc.4634.
APA：
Liu, Yang,Zhang, Dongjie,Du, Juan,Qin, Ying,Zhao, Zhigang...&Liu, Yi.(2019).Simultaneous determination of plasma nicotine and cotinine by UHPLC-MS/MS in C57BL/6 mice and its application in a pharmacokinetic study.BIOMEDICAL CHROMATOGRAPHY,33,(10)
MLA：
Liu, Yang,et al."Simultaneous determination of plasma nicotine and cotinine by UHPLC-MS/MS in C57BL/6 mice and its application in a pharmacokinetic study".BIOMEDICAL CHROMATOGRAPHY 33..10(2019)
