机构：[1]Department of Endocrinology, Shanghai Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China[2]Department of Endocrinology and Metabolism, Huai'an Second People's Hospital and The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China
Although diabetes mellitus (DM) is reported as an independent risk factor for colorectal cancer (CRC) in many researches, the underlying pathophysiology is still unclear. We investigated the differentially expressed genes (DEGs) for the diabetes and CRC to reveal the underlying pathophysiological association between the type 2-diabetic (T2D) and CRC. Gene expression profiles for T2D (GSE55650), CRC (GSE8671), and Metformin treated cell lines (GSE67342) were downloaded from GEO database. The DEGs between T2D samples and their control samples were identified with t-test and variance analysis. After cluster analysis and functional enrichment analysis, protein-protein interaction (PPI) network was constructed to find potential genes for diabetes and CRC in Metformin's treatment. Totally, we identified 583 overlapped genes, 169 common DEGs, and 414 independent DEGs between T2D and CRC samples. The common genes contained 89 up-regulated (DEGs1) and 80 down-regulated genes (DEGs3); and independent DEGs contained 270 down-regulated genes (DEGs4) in diabetes and 144 down-regulated genes (DEGs2) in CRC. In enrichment analysis, the Ribosome pathway was significantly enriched by the independent DEGs. The common genes were mainly enriched in some inflammatory related pathways. Two target genes of Metformin were significantly interacted with six hub genes (HADHB, NDUFS3, TAF1, MYC, HNFF4A, and MAX) with significant changes in expression values (P<0.05, t-test). To summary, it is suggested that the six hub genes might play important roles in the process of Metformin treatment for diabetes and CRC. However, specific pathology remains to be further studied.
基金：
Key Program of Science and Technology
Committee of Changning District,
Grant number: WKW2016 Z03
第一作者机构：[1]Department of Endocrinology, Shanghai Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Endocrinology, Shanghai Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China[*1]Department of Gastroenterology, Shanghai Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
推荐引用方式(GB/T 7714)：
Peng Wen-Fang,Bai Feng,Shao Kan,et al.The key genes underlying pathophysiology association between the type 2-diabetic and colorectal cancer[J].JOURNAL OF CELLULAR PHYSIOLOGY.2018,233(11):8551-8557.doi:10.1002/jcp.26440.
APA：
Peng, Wen-Fang,Bai, Feng,Shao, Kan,Shen, Li-Sha,Li, Hui-Hua&Huang, Shan.(2018).The key genes underlying pathophysiology association between the type 2-diabetic and colorectal cancer.JOURNAL OF CELLULAR PHYSIOLOGY,233,(11)
MLA：
Peng, Wen-Fang,et al."The key genes underlying pathophysiology association between the type 2-diabetic and colorectal cancer".JOURNAL OF CELLULAR PHYSIOLOGY 233..11(2018):8551-8557
