Inner hair cells (IHCs) and outer hair cells (OHCs) are the two anatomically and functionally distinct types of mechanosensitive receptor cells in the mammalian cochlea. The molecular mechanisms defining their morphological and functional specializations are largely unclear. As a first step to uncover the underlying mechanisms, we examined the transcriptomes of IHCs and OHCs isolated from adult CBA/J mouse cochleae. One thousand IHCs and OHCs were separately collected using the suction pipette technique. RNA sequencing of IHCs and OHCs was performed and their transcriptomes were analyzed. The results were validated by comparing some IHC and OHC preferentially expressed genes between present study and published microarray-based data as well as by real-time qPCR. Antibody-based immunocytochemistry was used to validate preferential expression of SLC7A14 and DNM3 in IHCs and OHCs. These data are expected to serve as a highly valuable resource for unraveling the molecular mechanisms underlying different biological properties of IHCs and OHCs as well as to provide a road map for future characterization of genes expressed in IHCs and OHCs.
基金：
NIH from the NIDCD [R01 DC016807]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81600798, 81770996]; Bellucci Depaoli Family Foundation; NCRRUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Center for Research Resources (NCRR) [1S10RR027754-01, 5P20RR016469, RR018788-08]; National Institute for General Medical Science (NIGMS) [8P20GM103427, P30GM110768]; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [P30GM110768, P20GM103427] Funding Source: NIH RePORTER; NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute on Deafness & Other Communication Disorders (NIDCD) [R01DC016807] Funding Source: NIH RePORTER
第一作者机构：[1]Beijing Capital Med Univ, Beijing Tongren Hosp, Dept Otorhinolaryngol, Beijing 100730, Peoples R China[2]Creighton Univ, Sch Med, Dept Biomed Sci, Omaha, NE 68170 USA
通讯作者：
通讯机构：[1]Beijing Capital Med Univ, Beijing Tongren Hosp, Dept Otorhinolaryngol, Beijing 100730, Peoples R China[2]Creighton Univ, Sch Med, Dept Biomed Sci, Omaha, NE 68170 USA
推荐引用方式(GB/T 7714)：
Li Yi,Liu Huizhan,Giffen Kimberlee P.,et al.Data Descriptor: Transcriptomes of cochlear inner and outer hair cells from adult mice[J].SCIENTIFIC DATA.2018,5:doi:10.1038/sdata.2018.199.
APA：
Li, Yi,Liu, Huizhan,Giffen, Kimberlee P.,Chen, Lei,Beisel, Kirk W.&He, David Z. Z..(2018).Data Descriptor: Transcriptomes of cochlear inner and outer hair cells from adult mice.SCIENTIFIC DATA,5,
MLA：
Li, Yi,et al."Data Descriptor: Transcriptomes of cochlear inner and outer hair cells from adult mice".SCIENTIFIC DATA 5.(2018)
