机构：[1]State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China[2]Department of Obstetrics and Gynecology, Tongren Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200336, China
Intracellular reactive oxygen species (ROS) accumulation during culture process compromise cord blood-derived hematopoietic stem/progenitor cells (CB-HSPCs) proliferation potential dramatically. In this work, the effects of hyperoside (Hyp) on CD34(+) cells were investigated by the analysis of cell expansion and secondary expansion potential, with the objective to optimize the culture conditions for ex vivo expansion of HSPCs. The results showed that the expansion of total cells treated with 1 mu M Hyp reached 54.9 +/- 9.6 folds, which was significantly higher than that of control cells (42.0 +/- 8.1 folds, P < 0.05), however, the percentages of CD34(+) cells and CD34(+) CD38(-) cells were similar in two cultures, leading to significantly higher expansion of CD34(+) cells and CD34(+) CD38(-) cells in Hyp-treated cultures (P < 0.05). Importantly, it was found that CD34(+) cells cultured with Hyp possessed better secondary expansion ability and colony forming potential, which meant better self-renewal potential. Furthermore, the addition of 1 mu M Hyp reduced the ROS level in total cells, CD34(+) cells and CD34(+) CD38(-) cells, and protected cells from apoptosis (P < 0.05). Together, Hyp is beneficial for the ex vivo expansion of functional HSPCs, which can be possibly related to its capability of downregulating the intracellular level of ROS.
基金：
Science and Technology Innovation Action Plan of Basic Research, Shanghai, China [15JC1401402]
第一作者机构：[1]State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
通讯作者：
通讯机构：[1]State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China[2]Department of Obstetrics and Gynecology, Tongren Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200336, China[*1]State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P. O. Box 309#, Shanghai 200237, China.[*2]Department of Obstetrics and Gynecology, Tongren Hospital Affiliated to Shanghai Jiaotong University School of Medicine, 1111 Xianxia Road, Shanghai 200336, China.
推荐引用方式(GB/T 7714)：
Zhang Wenyan,Zhang Weiwei,Zhang Xu,et al.Hyperoside promotes ex vivo expansion of hematopoietic stem/progenitor cells derived from cord blood by reducing intracellular ROS level[J].PROCESS BIOCHEMISTRY.2018,72:143-151.doi:10.1016/j.procbio.2018.06.016.
APA：
Zhang, Wenyan,Zhang, Weiwei,Zhang, Xu,Lu, Qin,Cai, Haibo&Tan, Wen-Song.(2018).Hyperoside promotes ex vivo expansion of hematopoietic stem/progenitor cells derived from cord blood by reducing intracellular ROS level.PROCESS BIOCHEMISTRY,72,
MLA：
Zhang, Wenyan,et al."Hyperoside promotes ex vivo expansion of hematopoietic stem/progenitor cells derived from cord blood by reducing intracellular ROS level".PROCESS BIOCHEMISTRY 72.(2018):143-151
