机构：[1]School of Ophthalmology and Optometry and Eye Hospital, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, China,[2]School of Basic Medical Science, Wenzhou Medical University, Wenzhou, China,[3]Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China研究所眼科研究所首都医科大学附属北京同仁医院首都医科大学附属同仁医院
Cdr1as is the abundant circular RNA (circRNA) in human and vertebrate retinas. However, the role of Cdr1as in the retina remains unknown. In this study, we aimed to generate a Cdr1as knockout (KO) mouse model and investigate the retinal consequences of Cdr1as loss of function. Through in situ hybridization (ISH), we demonstrated that Cdr1as is mainly expressed in the inner retina. Using CRISPR/Cas9 targeting Cdr1as, we successfully generated KO mice. We carried out ocular examinations in the KO mice until postnatal day 500. Compared with the age-matched wild-type (WT) siblings, the KO mice displayed increased b-wave amplitude of photopic electrophysiological response and reduced vision contrast sensitivity. Through small RNA profiling of the retinas, we determined that miR-7 was downregulated, while its target genes were upregulated. Taken together, our results demonstrated for the first time that Cdr1as ablation led to a mild retinal consequence in mice, indicating that Cdr1as abundance is not indispensable for retinal development and maintenance.
基金：
This study was supported by the National Key R&D Program
of China (2017YFA0105300) and Natural Science Foundation of
China (81870690 and 81970838).
第一作者机构：[1]School of Ophthalmology and Optometry and Eye Hospital, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, China,
通讯作者：
通讯机构：[1]School of Ophthalmology and Optometry and Eye Hospital, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, China,[2]School of Basic Medical Science, Wenzhou Medical University, Wenzhou, China,[3]Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China
推荐引用方式(GB/T 7714)：
Chen Xue-Jiao,Li Meng-Lan,Wang Ya-Han,et al.Abundant Neural circRNA Cdr1as Is Not Indispensable for Retina Maintenance[J].FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY.2020,8:doi:10.3389/fcell.2020.565543.
APA：
Chen, Xue-Jiao,Li, Meng-Lan,Wang, Ya-Han,Mou, Hao,Wu, Zhen...&Jin, Zi-Bing.(2020).Abundant Neural circRNA Cdr1as Is Not Indispensable for Retina Maintenance.FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,8,
MLA：
Chen, Xue-Jiao,et al."Abundant Neural circRNA Cdr1as Is Not Indispensable for Retina Maintenance".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 8.(2020)
