Increased H2S and its synthases in urothelial cell carcinoma of the bladder, and enhanced cisplatin-induced apoptosis following H2S inhibition in EJ cells
机构：[1]Beijing Chao Yang Hosp, Dept Urol, 8 Gongren Tiyuchang Nanlu, Beijing 100020, Peoples R China[2]Tianjin First Cent Hosp, Dept Urol, Tianjin 300191, Peoples R China
H2S, synthesized by cystathionine beta-synthase (CBS), cystathionine gamma-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (MPST), functions as a signalling molecule in mammalian cells. H2S serves complex functions in physiological and pathological processes, including in bladder cancer. In the present study, H2S production, the expression of the associated enzymes and the effect of H2S on human urothelial cell carcinoma of the bladder (UCB) tissue and cell lines were evaluated, and whether decreasing H2S levels influenced cell viability and tumour growth following treatment with cisplatin (CDDP) was assessed in UCB cells in vitro and in vivo. H2S production and the expression of CBS, CSE and MPST in bladder tissue specimens and the UCB cell lines 5637, EJ and UM-UC-3 were analysed using a sulfur-sensitive electrode and western blotting. UCB cells were subjected to different treatments, and viability and protein expression were determined. H2S production was inhibited to examine its influence on EJ cell tumour growth following CDDP treatment in vivo. It was identified that CBS, CSE and MPST protein were up-regulated in UCB tissues and cells. The H2S production and enzyme expression levels were the highest in UCB tissue and EJ cells. The inhibition of endogenous H2S biosynthesis decreased EJ cell viability and tumour growth in response to CDDP treatment. H2S levels and the associated biosynthetic enzymes were increased in human UCB tissue and cells compared with adjacent tissue and normal cells, which may have increased the resistance to CDDP-induced apoptosis in UCB. Therefore, H2S and its production may be an alternative therapeutic target for UCB. The inhibition of endogenous H2S biosynthesis decreased EJ cell viability and tumour growth in response to CDDP treatment. H2S levels and the associated biosynthetic enzymes were increased in human UCB tissue and cells compared with adjacent tissue and normal cells, which may have increased the resistance to CDDP-induced apoptosis in UCB. Therefore, H2S and its production may be an alternative therapeutic target for UCB.
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外文
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出版当年[2017]版：
大类|4 区医学
小类|4 区肿瘤学
最新[2025]版：
大类|4 区医学
小类|4 区肿瘤学
第一作者：
第一作者机构：[1]Beijing Chao Yang Hosp, Dept Urol, 8 Gongren Tiyuchang Nanlu, Beijing 100020, Peoples R China
通讯作者：
通讯机构：[1]Beijing Chao Yang Hosp, Dept Urol, 8 Gongren Tiyuchang Nanlu, Beijing 100020, Peoples R China[*1]Beijing Chao Yang Hosp, Dept Urol, 8 Gongren Tiyuchang Nanlu, Beijing 100020, Peoples R China
推荐引用方式(GB/T 7714)：
Wahafu Wasilijiang,Gai Junwei,Song Liming,et al.Increased H<sub>2</sub>S and its synthases in urothelial cell carcinoma of the bladder, and enhanced cisplatin-induced apoptosis following H<sub>2</sub>S inhibition in EJ cells[J].ONCOLOGY LETTERS.2018,15(6):8484-8490.doi:10.3892/ol.2018.8373.
APA：
Wahafu, Wasilijiang,Gai, Junwei,Song, Liming,Ping, Hao,Wang, Mingshuai...&Xing, Nianzeng.(2018).Increased H<sub>2</sub>S and its synthases in urothelial cell carcinoma of the bladder, and enhanced cisplatin-induced apoptosis following H<sub>2</sub>S inhibition in EJ cells.ONCOLOGY LETTERS,15,(6)
MLA：
Wahafu, Wasilijiang,et al."Increased H<sub>2</sub>S and its synthases in urothelial cell carcinoma of the bladder, and enhanced cisplatin-induced apoptosis following H<sub>2</sub>S inhibition in EJ cells".ONCOLOGY LETTERS 15..6(2018):8484-8490
