机构：[1]Chinese Acad Med Sci, Inst Basic Med Sci, Dept Biophys & Struct Biol, Beijing 100005, Peoples R China[2]Peking Union Med Coll, Sch Basic Med, Beijing 100005, Peoples R China[3]Heze Municipal Hosp, Dept Clin Lab, Yuan 274031, Shandong, Peoples R China[4]Xi An Jiao Tong Univ, Affiliated Hosp 1, Dept Oncol Surg, Xian 710061, Peoples R China[5]Jilin Univ, China Japan Union Hosp, Lab Med Ctr, Changchun 130041, Peoples R China
Interest in the pathophysiological role of IgG fragment crystallizable (Fc) N-linked glycosylation arose from changes in humoral immune responses. In this study, circulating disease-specific IgG (DSIgG) derived from serum immunoinflammation-related protein complexes was isolated from 846 serum samples of 443 patients with benign gastric diseases (BGDs) and 403 patients with gastric cancer (GC), and DSIgG glycopeptides attached to IgG Fc region at the site of Asn297 were analyzed using matrix-assisted laser desorption/ionization- Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR MS). A total of 22 glycopeptides were detected. Statistical analysis indicated that DSIgG1 G1S, DSIgG2 G0F, G1, G2F, and G2FS as well as DSIgG2 galactosylation and sialylation are significantly associated with sex in BGD patients and that the age-specific glycoforms and glycosylation features from DSIgG between BGD patients and GC patients have similar change trends. In addition, significant changes in galactosylation, sialylation, and bisecting N-acetylglucosamine (GlcNAc) from DSIgG were also observed between two pathophysiological states. Receiver operating characteristic (ROC) analysis indicated that the G2FN/G1FN (from DSIgG2) ratio has an excellent capability to distinguish female BGD patients from female GC patients over the age range of 20-79 years, with the sensitivity of 82.6%, the specificity of 82.6%, and the area under curve (AUC) of 0.872.
基金：
National Science Foundation of China [21575164]
基金编号：21575164
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|2 区综合性期刊
小类|2 区综合性期刊
最新[2025]版：
大类|3 区综合性期刊
小类|3 区综合性期刊
第一作者：
第一作者机构：[1]Chinese Acad Med Sci, Inst Basic Med Sci, Dept Biophys & Struct Biol, Beijing 100005, Peoples R China[2]Peking Union Med Coll, Sch Basic Med, Beijing 100005, Peoples R China
通讯作者：
通讯机构：[1]Chinese Acad Med Sci, Inst Basic Med Sci, Dept Biophys & Struct Biol, Beijing 100005, Peoples R China[*1]Chinese Acad Med Sci, Inst Basic Med Sci, Dept Biophys & Struct Biol, Beijing 100005, Peoples R China[2]Peking Union Med Coll, Sch Basic Med, Beijing 100005, Peoples R China[*2]Peking Union Med Coll, Sch Basic Med, Beijing 100005, Peoples R China
推荐引用方式(GB/T 7714)：
Zhang Dan,Chen Bingchao,Wang Yanmin,et al.Disease-specific IgG Fc N-glycosylation as personalized biomarkers to differentiate gastric cancer from benign gastric diseases[J].SCIENTIFIC REPORTS.2016,6:25957.doi:10.1038/srep25957.
APA：
Zhang, Dan,Chen, Bingchao,Wang, Yanmin,Xia, Peng,He, Chengyan...&Li, Zhili.(2016).Disease-specific IgG Fc N-glycosylation as personalized biomarkers to differentiate gastric cancer from benign gastric diseases.SCIENTIFIC REPORTS,6,
MLA：
Zhang, Dan,et al."Disease-specific IgG Fc N-glycosylation as personalized biomarkers to differentiate gastric cancer from benign gastric diseases".SCIENTIFIC REPORTS 6.(2016)
