机构：[1]Department of Orthopedics, the Second Hospital, Shanxi Medical University, Taiyuan, Shanxi, China[2]Department of Orthopedics, Warren Alpert Medical School of Brown University/Rhode Island Hospital, Providence RI, USA[3]Department of Orthopedics, Sir Run Run Shaw Hospital, Medical School of Zhejiang University, Hangzhou, Zhejiang, China[4]Foot and Ankle Orthopaedic Surgery Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China首都医科大学附属北京同仁医院首都医科大学附属同仁医院
Western blot of synovial fluid has been widely used for osteoarthritis (OA) research and diagnosis, but there is no ideal loading control for this purpose. Although β-actin is extensively used as loading control in western blot, it is not suitable for synovial fluid because it is not required in synovial fluid as a cytoskeletal protein. A good loading control for synovial fluid in OA studies should have unchanged content in synovial fluids from normal and OA groups, because synovial fluid protein content can vary with changes in synovial vascular permeability with OA onset. In this study, we explore the potential of using α1-antitripsin (A1AT) as loading control for OA synovial fluid in western blot. A1AT level is elevated in inflammatory conditions such as rheumatoid arthritis (RA). Unlike RA, OA is a non-inflammation disease, which does not induce A1AT. In this study, we identified A1AT as an abundant component of synovial fluid by Mass Spectrometry and confirmed that the level of A1AT is relative constant between human OA and normal synovial fluid by western blot and ELISA. Hence, we proposed that A1AT may be a good loading control for western blot in human OA synovial fluid studies provided that pathological conditions such as RA or A1AT deficiency associated liver or lung diseases are excluded.
基金：
NIH (National Institute of Arthritis
and Musculoskeletal and Skin Diseases grant R01-AR-059142,
National Institute of General Medical Sciences grant R01-CA-166089,
and National Center for Research Resources grant P20-RR-024484)
and the National Natural Science Foundation of China (grants
81071495, 81171676, and 81201435).
语种：
外文
PubmedID：
第一作者：
第一作者机构：[1]Department of Orthopedics, the Second Hospital, Shanxi Medical University, Taiyuan, Shanxi, China[2]Department of Orthopedics, Warren Alpert Medical School of Brown University/Rhode Island Hospital, Providence RI, USA[3]Department of Orthopedics, Sir Run Run Shaw Hospital, Medical School of Zhejiang University, Hangzhou, Zhejiang, China[*1]No.382 Wuyi Road, Taiyuan, Shanxi, 030001, China
通讯作者：
通讯机构：[1]Department of Orthopedics, the Second Hospital, Shanxi Medical University, Taiyuan, Shanxi, China[2]Department of Orthopedics, Warren Alpert Medical School of Brown University/Rhode Island Hospital, Providence RI, USA[3]Department of Orthopedics, Sir Run Run Shaw Hospital, Medical School of Zhejiang University, Hangzhou, Zhejiang, China[*1]No.382 Wuyi Road, Taiyuan, Shanxi, 030001, China
推荐引用方式(GB/T 7714)：
Wang Shaowei,Zhou Jingming,Wei Xiaochun,et al.Identification of α1-Antitrypsin as a Potential Candidate for Internal Control for Human Synovial Fluid in Western Blot.[J].Rheumatology (Sunnyvale, Calif.).2015,Suppl 6:006.doi:10.4172/2161-1149.S6-006.
APA：
Wang Shaowei,Zhou Jingming,Wei Xiaochun,Li Pengcui,Li Kai...&Wei Lei.(2015).Identification of α1-Antitrypsin as a Potential Candidate for Internal Control for Human Synovial Fluid in Western Blot..Rheumatology (Sunnyvale, Calif.),Suppl 6,
MLA：
Wang Shaowei,et al."Identification of α1-Antitrypsin as a Potential Candidate for Internal Control for Human Synovial Fluid in Western Blot.".Rheumatology (Sunnyvale, Calif.) Suppl 6.(2015):006
