机构:[1]Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin, 300384, PR China[2]Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology &Visual Sciences, Beijing Institute of Ophthalmology, Beijing, 100730, PR China研究所眼科研究所首都医科大学附属北京同仁医院首都医科大学附属同仁医院
Meibomian gland carcinoma (MGC) is a malignant eyelid tumor with a high malignancy degree and poor prognosis. However, the lack of suitable cell and animal models has limited the study of MGC pathogenesis. In the present study, we established and identified one human MGC cell and one meibomian gland (MG) cell model by fresh surgical resection tissue block primary culture and differentially expressed gene assays. The outgrowth of MGC and MG cells was periodically observed after primary culture, and the first passage of MGC cells proceeded on the 14th day, whereas that for MG cells after three weeks. Cell ultrastructures were observed by transmission electron microscopy (TEM). Immunofluorescence staining showed that MGC and MG cells were both positive for cytokeratin (CK) and androgen receptor (AR). Orange granules were observed in the cytoplasm of MGC and MG cells using Oil red O staining, but they were stronger for MG cells than for MGC. CCK-8 detection demonstrated that the proliferation ability of MGC cells was stronger than that of MG cells. Moreover, during RNA sequence analyses, 3023 differential expressed genes were detected between MGC and MG cells. These genes were involved in biological processes such as cell division and positive regulation of cell migration; the signaling pathways mainly covered cell cycle and DNA replication. Further, the tumorigenic potential of MGC cells was examined by inoculating them subcutaneously into the right abdomen of three severely immunodeficient NOD-SCID mice. Transplanted tumors formed on day 11 after inoculation. The xenograft mouse tissues retained the same histological characteristics as the human MGC original tumor and MGC primary cells. Altogether, these results showed that the MGC and MG models were successfully cultured and established, and differentially expressed genes were successfully detected. We provided a useful model and molecular basis for studying the biological characteristics and pathogenesis of human MGC.
基金:
The National Natural Science Foundation of China (grant no. 81570872 and 81800825) and The Tianjin Municipal Health Commission (grant no. MS20025).
第一作者机构:[1]Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin, 300384, PR China
共同第一作者:
通讯作者:
通讯机构:[1]Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin, 300384, PR China[*1]Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, 251 Fu Kang Road, Tianjin, 300384, PR China.
推荐引用方式(GB/T 7714):
Zhang Chuanli,Zhu Limin,Liu Xun,et al.Establishment of a human meibomian gland carcinoma cell model and analysis of differently expressed genes[J].EXPERIMENTAL EYE RESEARCH.2022,219:doi:10.1016/j.exer.2022.108983.
APA:
Zhang, Chuanli,Zhu, Limin,Liu, Xun,Jiang, Meixia,Tang, Qin...&He, Yanjin.(2022).Establishment of a human meibomian gland carcinoma cell model and analysis of differently expressed genes.EXPERIMENTAL EYE RESEARCH,219,
MLA:
Zhang, Chuanli,et al."Establishment of a human meibomian gland carcinoma cell model and analysis of differently expressed genes".EXPERIMENTAL EYE RESEARCH 219.(2022)