Allergic asthma is an allergic inflammatory disease of the airways, in which numerous cell types and cytokines have been shown to contribute to pathogenesis of the disease. Although increased expression of IL-9 has been shown to influence the activity of structural as well as eosinophils and mast cells in asthma, the influence of IL-9 on function of ILC2 and Th2 cells remains unclear. This study therefore aimed to elucidate the role of IL-9 on ILC2 and Th2 cells using a murine model of asthma. A murine model of asthma was established using wild type (WT) and IL-9-deficient (Il9(-/-)) transgenic mice sensitized to house dust mite (HDM). Bronchoalveolar lavage fluid (BALF) and lung tissues were collected, and analysed for inflammatory cells (eosinophils, mast cells, Th2 cells and ILC2 cells), histopathological changes, and several cytokines. HDM challenge significantly increased accumulation of ILC2 cells, Th2 cells and mast cells, as well as goblet cell hyperplasia, and the expression of cytokines IL-4, IL-5 and IL-13, but not IFN-gamma, in WT mice compared to saline-challenged control group. In contrast, all pathological changes, including infiltration of ILC2 cells, Th2 cells and mast cells, were significantly attenuated in HDM-challenged Il9(-/-) mice. Furthermore, the number of Ki67(+)ILC2 cells, Ki67(+)Th2 cells and Ki67(+)mast cells were significantly reduced in the absence of IL-9 signalling. These data suggest that IL-9 promotes the proliferation and type 2 cytokine production of type 2 cells in the murine models of asthma, and therefore might be a potential therapeutic target for asthma treatment.
基金：
National Natural Science Foundation of China [81700026]; program for the Changjiang scholars and innovative research team [IRT13082]; CAMS Innovation Fund for Medical Sciences [2019-I2M-5-022]; Beijing municipal science and technology project [Z181100001618002]; Public Welfare Development and Reform Pilot Project [2019-10]; Beijing Municipal Administration of Hospital's Youth Programme [QML20180201]; The priming scientific research foundation for the junior researcher in Beijing Tongren Hospital, Capital Medical University
第一作者机构：[1]Capital Med Univ, Beijing Tongren Hosp, Beijing Inst Otorhinolaryngol,Beijing Key Lab Nas, Dept Otorhinolaryngol Head & Neck Surg,Minist Edu, 17 HouGouHuTong, Beijing 100730, Peoples R China
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推荐引用方式(GB/T 7714)：
Li Yan,Lan Feng,Yang Yiran,et al.The absence of IL-9 reduces allergic airway inflammation by reducing ILC2, Th2 and mast cells in murine model of asthma[J].BMC PULMONARY MEDICINE.2022,22(1):doi:10.1186/s12890-022-01976-2.
APA：
Li, Yan,Lan, Feng,Yang, Yiran,Xu, Yingjie,Chen, Yalin...&Zhang, Luo.(2022).The absence of IL-9 reduces allergic airway inflammation by reducing ILC2, Th2 and mast cells in murine model of asthma.BMC PULMONARY MEDICINE,22,(1)
MLA：
Li, Yan,et al."The absence of IL-9 reduces allergic airway inflammation by reducing ILC2, Th2 and mast cells in murine model of asthma".BMC PULMONARY MEDICINE 22..1(2022)
