机构：[1]Department of Pharmacy, Shanghai University of Medicine and Health Sciences, Shanghai, China,[2]Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai, China,[3]Tongren Hospital and State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacology and Chemical Biology, Hongqiao International Institute of Medicine, Shanghai Jiao Tong University School of Medicine (SJTU-SM), Shanghai, China,[4]Department of General Surgery, Tongren Hospital, SJTU-SM, Shanghai, China
Saikosaponin A (SSA), a main triterpenoid saponin component from Radix Bupleurum, has been revealed to have a variety of pharmacological activities. However, whether SSA can inhibit angiogenesis, a key step in solid tumor progression, remains unknown. In this study, we demonstrated that SSA could powerfully suppress the proliferation, migration, and tube formation of human umbilical vein endothelial cells. SSA also significantly inhibited angiogenesis in the models of the chick embryo chorioallantoic membrane and Matrigel plugs. Moreover, SSA was found to inhibit tumor growth in both orthotopic 4T1 breast cancer and subcutaneous HCT-15 colorectal tumor by the inhibition of tumor angiogenesis. Western blot assay indicated the antiangiogenic mechanism of SSA in the suppression of the protein phosphorylation of VEGFR2 and the downstream protein kinase including PLC gamma 1, FAK, Src, and Akt. In summary, SSA can suppress angiogenesis and tumor growth by blocking the VEGFR2-mediated signaling pathway.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81602729, 81773274, 82073379]
第一作者机构：[1]Department of Pharmacy, Shanghai University of Medicine and Health Sciences, Shanghai, China,[2]Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai, China,
共同第一作者：
通讯作者：
通讯机构：[1]Department of Pharmacy, Shanghai University of Medicine and Health Sciences, Shanghai, China,[2]Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai, China,
推荐引用方式(GB/T 7714)：
Zhang Pan,Lai Xing,Zhu Mao-Hua,et al.Saikosaponin A, a Triterpene Saponin, Suppresses Angiogenesis and Tumor Growth by Blocking VEGFR2-Mediated Signaling Pathway[J].FRONTIERS IN PHARMACOLOGY.2021,12:doi:10.3389/fphar.2021.713200.
APA：
Zhang, Pan,Lai, Xing,Zhu, Mao-Hua,Long, Mei,Liu, Xue-Liang...&Zhao, Mei.(2021).Saikosaponin A, a Triterpene Saponin, Suppresses Angiogenesis and Tumor Growth by Blocking VEGFR2-Mediated Signaling Pathway.FRONTIERS IN PHARMACOLOGY,12,
MLA：
Zhang, Pan,et al."Saikosaponin A, a Triterpene Saponin, Suppresses Angiogenesis and Tumor Growth by Blocking VEGFR2-Mediated Signaling Pathway".FRONTIERS IN PHARMACOLOGY 12.(2021)
