机构：[1]Department of Neonatology, Dongguan Children's Hospital Affiliated to Guangdong Medical University, Dongguan, China.[2]Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.[3]Department of Neonatology, International Peace Maternity and Child Health Hospital of China Welfare Institution, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.[4]Department of Neonatology, Nanjing Children's Hospital (Children's Hospital Affiliated to Nanjing Medical University), Nanjing, China.
Bronchopulmonary dysplasia (BPD) is a chronic lung disease that occurs in preterm infants and lacks effective treatment. We aim to reveal the relationship between amniotic fluid (AF) peptides and lung development by analyzing the differences in the composition of AF peptides at different gestational periods, thus providing a new means of prevention and treatment for BPD.Based on the stages of lung development, we collected AF by amniocentesis in two different gestational periods, using the 25th week of pregnancy as the cut-off. We conducted a peptide omics analysis of these AF samples using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Additionally, we verified the regulatory effects of hyperoxia and the peptide COL5A2 on BPD-related cells [(mouse lung epithelial (MLE-12) cells] by 5-Ethynyl-2'-deoxyuridine (EdU) staining, JC-1 staining, flow cytometry, and reactive oxygen species (ROS) assay.There were 131 differentially expressed peptides, including 85 up-regulated and 46 down-regulated [fold change (FC) ≥1.2 or ≤1/1.2, P<0.05], in the ≥25 weeks' gestation group compared to the <25 weeks' gestation group. Further bioinformatics analysis revealed that the precursor proteins of the differentially expressed peptides between these two groups were involved in the regulation of the developmental process, anatomical structure development, and other biological processes, suggesting that these differential peptides may play a key role in lung development. We found peptide COL5A2 with the sequence GPPGEPGPPG and verified the regulatory effects of COL5A2 on the proliferation, apoptosis, cell viability, and ROS levels of MLE-12 cells by cell assays.In this study, peptidomic studies using AF from different gestational periods revealed that peptides in AF may be involved in lung development. They could be used in the future to assist in the postnatal development of preterm infants and provide new therapeutic prospects for BPD.2023 Translational Pediatrics. All rights reserved.
基金：
Social Science
and Technology Development Project of Dongguan, China
(No. 20221800905362), and by grants from the National
Natural Science Foundation of China (No. 81901512 to
Xingyun Wang) and Shanghai Rising-Star Program (No.
22QB1401000 to Xingyun Wang).
第一作者机构：[1]Department of Neonatology, Dongguan Children's Hospital Affiliated to Guangdong Medical University, Dongguan, China.[2]Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.[3]Department of Neonatology, International Peace Maternity and Child Health Hospital of China Welfare Institution, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Neonatology, Dongguan Children's Hospital Affiliated to Guangdong Medical University, Dongguan, China.[2]Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.[*1]Dongguan Children’s Hospital Affiliated to Guangdong Medical University, Dongguan, China[*2]Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
推荐引用方式(GB/T 7714)：
Li Baihe,Chen Xu,Ma Weihui,et al.An endogenous amniotic fluid-derived 10-amino acid peptide improves lung development and hyperoxia injury[J].TRANSLATIONAL PEDIATRICS.2023,12(1):46-+.doi:10.21037/tp-22-681.
APA：
Li Baihe,Chen Xu,Ma Weihui,Li Ning,Chen Qin...&Xu Fengdan.(2023).An endogenous amniotic fluid-derived 10-amino acid peptide improves lung development and hyperoxia injury.TRANSLATIONAL PEDIATRICS,12,(1)
MLA：
Li Baihe,et al."An endogenous amniotic fluid-derived 10-amino acid peptide improves lung development and hyperoxia injury".TRANSLATIONAL PEDIATRICS 12..1(2023):46-+
