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Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis

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机构: [1]College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China [2]Sichuan Friendship Hospital, Chengdu, China [3]Tong Ren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China [4]Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China
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关键词: Growth stimulating express gene 2 protein Time-resolved fluorescence immunoassay Sepsis Septic shock

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This study aimed to establish a highly sensitive time-resolved fluorescence immunoassay of growth stimulating express gene 2 protein (ST2-TRFIA) and evaluate its application value for sepsis.Two types of ST2 monoclonal specific antibodies against different epitopes of antigen molecule were used as coating and Eu3+-labeled antibodies. The double-antibody sandwich method was used in establishing ST2-TRFIA, and the methodology was evaluated. The established ST2-TRFIA was used in detecting ST2 concentration in the plasma samples of healthy controls and sepsis.The linear range of ST2-TRFIA was 1.446-500 ng/mL. Plasma ST2 concentrations detected through ST2-TRFIA were consistent with the results of fluorescence quantitative immunochromatography (ρ = 0.946). The plasma ST2 concentrations of patients with sepsis were significantly higher than those of healthy controls (P < 0.01).This study successfully established a highly sensitive ST2-TRFIA, which was highly comparable to commercially available fluorescent quantitative immunochromatographic kits and can facilitate the timely diagnosis of sepsis.Copyright © 2023. Published by Elsevier B.V.

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出版当年[2022]版:
大类 | 4 区 医学
小类 | 4 区 免疫学 4 区 生化研究方法
最新[2023]版:
大类 | 4 区 医学
小类 | 4 区 生化研究方法 4 区 免疫学
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出版当年[2021]版:
Q4 BIOCHEMICAL RESEARCH METHODS Q4 IMMUNOLOGY
最新[2023]版:
Q4 BIOCHEMICAL RESEARCH METHODS Q4 IMMUNOLOGY

影响因子: 最新[2023版] 最新五年平均 出版当年[2021版] 出版当年五年平均 出版前一年[2020版] 出版后一年[2022版]

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第一作者机构: [1]College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China
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通讯机构: [1]College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China [4]Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China [*1]Immunoassay Laboratory, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310016, China. [*2]Emergency and Critical Care Center, Department of Emergency Medicine, Zhejiang Provincial People's Hospital (Affiliated People's Hospital, Hangzhou Medical College), Hangzhou, Zhejiang 310014, China.
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