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Transcriptome sequencing and microRNA-mRNA regulatory network construction in the lens from a Na2SeO3-induced Sprague Dawley rat cataract model

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机构： [1]Beijing Tongren Hospital, Beijing, 100730, China. [2]Capital Medical University, Beijing, 100730, China. [3]The State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300071, China. [4]Beijing Tongren Eye Center, Beijing, China. [5]Beijing, Ophthalmology & Visual Sciences Key Lab, Beijing, China.

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关键词： mRNA Cataract microRNA Network Rat Len

摘要：

A sight-threatening, cataract is a common degenerative disease of the ocular lens. This study aimed to explore the regulatory mechanism of age-related cataract (ARC) formation and progression.Cataracts in Sprague Dawley rats were induced by adopting the method that injected selenite subcutaneously in the nape. We performed high-throughput RNA sequencing technology to identify the mRNA and microRNA(miRNA) expression profiles of the capsular membrane of the lens from Na2SeO3-induced and saline-injected Sprague Dawley rats. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were carried out to forecast the regulatory and functional role of mRNAs in cataracts by DAVID and Metascape. The protein-protein interaction(PPI) network of differentially expressed mRNA(DEmRNAs) was built via the STRING. Target miRNAs of hub genes were predicted by miRBD and TargetScan. Furthermore, differentially expressed miRNA(DEmiRNAs) were selected as hub genes' targets, validated by quantitative real-time polymerase chain reaction(qRT-PCR), and a DEmiRNA-DEmRNA regulatory network was constructed via Cytoscape.In total, 329 DEmRNAs including 40 upregulated and 289 downregulated genes were identified. Forty seven DEmiRNAs including 29 upregulated and 18 downregulated miRNAs were detected. The DEmRNAs are involved in lens development, visual perception, and aging-related biological processes. A protein-protein interaction network including 274 node genes was constructed to explore the interactions of DEmRNAs. Furthermore, a DEmiRNA-DEmRNA regulatory network related to cataracts was constructed, including 8 hub DEmRNAs, and 8 key DEmiRNAs which were confirmed by qRT-PCR analysis.We identified several differentially expressed genes and established a miRNA-mRNA-regulated network in a Na2SeO3-induced Sprague Dawley rat cataract model. These results may provide novel insights into the clinical treatment of cataracts, and the hub DEmRNAs and key DEmiRNAs could be potential therapeutic targets for ARC.© 2023. The Author(s).

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出版当年[2022]版：

大类 | 4 区医学

小类 | 4 区眼科学

最新[2025]版：

大类 | 3 区医学

小类 | 3 区眼科学

JCR分区：

出版当年[2021]版：

Q3 OPHTHALMOLOGY

最新[2023]版：

Q3 OPHTHALMOLOGY

影响因子： 1.7 最新[2023版] 2 最新五年平均 2.086 出版当年[2021版] 2.493 出版当年五年平均 2.209 出版前一年[2020版] 2 出版后一年[2022版]

第一作者：

第一作者机构： [1]Beijing Tongren Hospital, Beijing, 100730, China. [2]Capital Medical University, Beijing, 100730, China.

通讯作者：

通讯机构： [1]Beijing Tongren Hospital, Beijing, 100730, China. [2]Capital Medical University, Beijing, 100730, China. [4]Beijing Tongren Eye Center, Beijing, China. [5]Beijing, Ophthalmology & Visual Sciences Key Lab, Beijing, China.

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Transcriptome sequencing and microRNA-mRNA regulatory network construction in the lens from a Na2SeO3-induced Sprague Dawley rat cataract model

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