机构：[1]Departments of Otolaryngology, Head and Neck Surgery,Beijing TongRen Hospital, Capital Medical University首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]Departments of Allergy,Beijing TongRen Hospital, Capital Medical University首都医科大学附属北京同仁医院首都医科大学附属同仁医院[3]Departments of Pathology,Beijing TongRen Hospital, Capital Medical University首都医科大学附属北京同仁医院首都医科大学附属同仁医院[4]Department of Immunology, School of Basic Medical Sciences, Capital Medical University[5]the Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology研究所耳鼻咽喉科研究所首都医科大学附属北京同仁医院首都医科大学附属同仁医院[6]the Research Unit of Diagnosis and Treatment of Chronic Nasal Diseases, Chinese Academy of Medical Sciences,Beijing
Background: Whether IgE affects eosinophil migration in chronic rhinosinusitis with nasal polyps (CRSwNP) remains largely unclear. Moreover, our understanding of local IgE, eosinophils, and omalizumab efficacy in CRSwNP remains limited. Objective: We investigated whether IgE acts directly on eosinophils and determined its role in omalizumab therapy. Methods: Eosinophils and their surface receptors were detected by hematoxylin and eosin staining and flow cytometry. IgE and its receptors, eosinophil peroxidase (EPX), eosinophilic cationic protein, and CCR3 were detected by immunohistochemistry and immunofluorescence. Functional analyses were performed on blood eosinophils and polyp tissues. Logistic regression was performed to screen for risk factors. Receiver operating characteristic curve was generated to evaluate the accuracy. Results: Both Fc epsilon RI and CD23 were expressed on eosinophils. The expression of Fc epsilon RI and CD23 on eosinophil in nasal polyp tissue was higher than in peripheral blood (both P < .001). IgE and EPX colocalized in CRSwNP. IgE directly promoted eosinophil migration by upregulating CCR3 in CRSwNP but not in healthy controls. Omalizumab and lumiliximab were found to be effective in restraining this migration, indicating CD23 was involved in IgE-induced eosinophil migration. Both IgE(+) and EPX+ cells were significantly reduced after omalizumab treatment in those who experienced response (IgE(+) cells, P = .001; EPX+ cells, P = .016) but not in those with no response (IgE(+) cells, P = .060; EPX+ cells, P = .151). Baseline IgE(+) cell levels were higher in those with response compared to those without response (P = .024). The baseline local IgE(+ )cell count predicted omalizumab efficacy with an accuracy of 0.811. Conclusions: IgE directly promotes eosinophil migration, and baseline local IgE(+) cell counts are predictive of omalizumab efficacy in CRSwNP. (J Allergy Clin Immunol 2024;153:447-60.)
基金：
National Natural Science Foundation of China [82025010, 81630023, 82171108, 81900917, 81870698]; Beijing Municipal Administration of Hospitals' Mission Plan [SML20150203]; Beijing Munic- ipal Administration of Hospitals' Dengfeng Plan [DFL20190202]; Capital's Funds for Health Improvement and Research [2022-1-1091]
第一作者机构：[1]Departments of Otolaryngology, Head and Neck Surgery,Beijing TongRen Hospital, Capital Medical University[5]the Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology[6]the Research Unit of Diagnosis and Treatment of Chronic Nasal Diseases, Chinese Academy of Medical Sciences,Beijing
共同第一作者：
通讯作者：
通讯机构：[1]Departments of Otolaryngology, Head and Neck Surgery,Beijing TongRen Hospital, Capital Medical University[2]Departments of Allergy,Beijing TongRen Hospital, Capital Medical University[4]Department of Immunology, School of Basic Medical Sciences, Capital Medical University[5]the Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology[6]the Research Unit of Diagnosis and Treatment of Chronic Nasal Diseases, Chinese Academy of Medical Sciences,Beijing[*1]Beijing Institute of Otolaryngology, No. 17, Chongneihougou Hutong, Dongcheng District, Beijing 100005, China.[*2]Department of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, No. 1, Dongjiaominxiang Street, Dongcheng District, Beijing 100730, China.[*3]Capital Medical University, No. 10, Xitoutiao, Youanmenwai, Fengtai District, Beijing, China
推荐引用方式(GB/T 7714)：
Yu Jiaqi,Yan Bing,Shen Shen,et al.IgE directly affects eosinophil migration in chronic rhinosinusitis with nasal polyps through CCR3 and predicts the efficacy of omalizumab[J].JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY.2024,153(2):doi:10.1016/j.jaci.2023.09.041.
APA：
Yu, Jiaqi,Yan, Bing,Shen, Shen,Wang, Yang,Li, Ying...&Wang, Chengshuo.(2024).IgE directly affects eosinophil migration in chronic rhinosinusitis with nasal polyps through CCR3 and predicts the efficacy of omalizumab.JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY,153,(2)
MLA：
Yu, Jiaqi,et al."IgE directly affects eosinophil migration in chronic rhinosinusitis with nasal polyps through CCR3 and predicts the efficacy of omalizumab".JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY 153..2(2024)
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