This paper aims to investigate the possible effects of Syringaresinol on a myocardial ischemia/reperfusion injury cell model and uncover the underlying mechanism. A myocardial ischemia/reperfusion injury cell model was constructed using an oxygen-glucose deprivation/reperfusion-subjected H9c2 cell line. 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide) and lactate dehydrogenases releasing assays showed the effects on cell viability. Immunoblot and flow cytometry assays showed the impact on cell pyroptosis. Enzyme-linked immunosorbent assay uncovered the effects on oxidative stress in H9c2 cells. Further, immunoblot and quantitative polymerase chain reaction assays were conducted to confirm the mechanism. Syringaresinol enhanced the survival and suppressed pyroptosis in H9c2 cells subjected to oxygen-glucose deprivation/reperfusion. Syringaresinol also inhibited oxidative stress in H9c2 cells subjected to oxygen-glucose deprivation/reperfusion, and mechanically, it suppressed the expression of HSP90, thus inhibiting myocardial ischemia/reperfusion injury. Syringaresinol ameliorated the pyroptosis and oxidative stress of oxygen-glucose deprivation/reperfusion in cardiomyocytes and alleviated cardiomyocyte damage.