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Huazhi Rougan Granule Alleviates Liver and Intestinal Damage in Non-Alcoholic Fatty Liver Disease by Regulating miR-122 Expression and TLR4/MyD88/NF-κB Pathway Activation

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机构: [1]Department of Traditional Chinese Medicine, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan 430060, P. R, China. [2]Department of Integrated Traditional Chinese and Western Medicine, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Kunming 650019, Yunnan, China. [3]Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China.
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关键词: Non-alcoholic fatty liver miR-122 TLR4/MyD88/NF-κB pathway liver intestinal injury aspartate aminotransferase

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miR-122 is upregulated in non-alcoholic fatty liver disease (NAFLD) liver tissue, and knockdown of miR-122 protects hepatocytes from lipid metabolism disorders. This study aimed to investigate whether Huazhi Rougan Granule (HRG) alleviates NAFLD liver and intestinal injury by regulating the miR-122-mediated TLR4/MyD88/NF-κB pathway.Rats with NAFLD were constructed by high-fat feeding. Serum levels of total cholesterol (TC), triglycerides (TG), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were measured using a fully automated biochemical instrument. Histopathological changes in the liver and small intestine were observed by HE staining. QRT-PCR detected the expression level of miR-122 in the liver tissues. The protein expression of TLR4, MyD88, NF- κB p65, and p-p65 in liver tissues was detected by western blotting.HRG slowed down the weight gain of NAFLD rats, decreased (P<0.05) the levels of TC, TG, ALT, AST, TNF-α, IL-1β, IL-6, LPS, and Hpt, improved the pathological status of liver and small intestine tissues, upregulated (P<0.05) the expression of ZO-1 and Occludin, downregulated (P<0.05) the protein expression of TLR4, MyD88, and p-p65, and inhibited (P<0.05) the expression of miR-122.HRG may alleviate hepatic and intestinal injuries in rats with NAFLD by regulating the miR-122-mediated TLR4/MyD88/NF-κB pathway.Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.

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出版当年[2023]版:
大类 | 4 区 医学
小类 | 4 区 生化研究方法 4 区 应用化学 4 区 药学
最新[2025]版:
大类 | 4 区 医学
小类 | 4 区 生化研究方法 4 区 应用化学 4 区 药学
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第一作者机构: [1]Department of Traditional Chinese Medicine, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan 430060, P. R, China.
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通讯机构: [*1]Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China
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