机构：[1]Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China北京朝阳医院[2]Department of Neurology, Beijing First Hospital of Integrated Chinese and Western Medicine, Beijing 100000, China[3]Department of Emergency Medicine, Beijing Fuxing Hospital, Capital Medical University, Beijing 100038, China[4]Department of Emergency Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China首都医科大学附属北京友谊医院[5]Department of Emergency Medicine, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China临床科室急诊科首都医科大学附属北京同仁医院首都医科大学附属同仁医院
Background Our previous study found that mild hypothermia (MH) after resuscitation reduced cerebral microcirculation, but the mechanism was not elucidated. The aim of this study was to clarify changes of endothelin-1 (ET-1) and nitric oxide (NO) systems in brain tissue during hypothermia after resuscitation. Methods Twenty-six domestic male Beijing Landrace pigs were used in this study. MH was intravascularly induced 1 h after resuscitation from 8-min ventricular fibrillation. Core temperature was reduced to 33 degrees C and maintained until 8 h after resuscitation, and then animals were euthanized. ET-1 and NO levels in brain tissue and peripheral plasma were measured. Expression of endothelin-converting enzyme-1 (ECE-1), endothelin A receptor (ET-AR), endothelin-B receptor, and nitric oxide synthase (NOS) in brain tissue was determined by Western blot analysis. Results Compared with non-hypothermia (NH) treatment, MH after resuscitation significantly increased the level of endothelin-1 and reduced the level of NO in peripheral blood and brain tissue. Cerebral expression of ECE-1 and ET-AR was significantly increased during MH after resuscitation. Moreover, MH significantly decreased inducible NOS expression compared with the NH group. Conclusions The ET-1 system is activated, while inducible NOS is inhibited in brain tissue during MH after resuscitation.
基金：
National Natural Science Foundation of China
(No. 81801882) and Foundation for Training Excellent Talents of Beijing (No.
2017000021469G219).
第一作者机构：[1]Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
通讯作者：
推荐引用方式(GB/T 7714)：
Wu Junyuan,Li Zhiwei,Yuan Wei,et al.Changes of Endothelin-1 and Nitric Oxide Systems in Brain Tissue During Mild Hypothermia in a Porcine Model of Cardiac Arrest[J].NEUROCRITICAL CARE.2020,33(1):73-81.doi:10.1007/s12028-019-00855-9.
APA：
Wu, Junyuan,Li, Zhiwei,Yuan, Wei,Zhao, Yongzhen,Li, Jie...&Li, Chunsheng.(2020).Changes of Endothelin-1 and Nitric Oxide Systems in Brain Tissue During Mild Hypothermia in a Porcine Model of Cardiac Arrest.NEUROCRITICAL CARE,33,(1)
MLA：
Wu, Junyuan,et al."Changes of Endothelin-1 and Nitric Oxide Systems in Brain Tissue During Mild Hypothermia in a Porcine Model of Cardiac Arrest".NEUROCRITICAL CARE 33..1(2020):73-81
