Objective: The study was aimed to explore the hepatocellular protective functions of cafestol during hepatic ischemia-reperfusion injury and the possible mechanisms. Methods: Ninety male Balb/c mice were randomly divided into seven groups, including normal control group, L-cafestol(20mg/kg) group, H-cafestol(40mg/kg) group, sham group, IR group, L-cafestol(20mg/kg) + IR group, H-cafestol(40mg/kg) + IR group. Serum liver enzymes (ALT, AST), inflammation mediators, proteins associated with apoptosis and autophagy, indicators linked with ERK/PPAR gamma pathway, and liver histopathology were measured using ELISA, qRT-PCR, immunohistochemical staining, and western blotting at 2, 8, and 24 hours after reperfusion. Results: Our findings confirmed that cafestol preconditioning groups could reduce the levels of ALT and AST, alleviate liver pathological damage, suppress the release of inflammation mediators, inhibit the production of pro-apoptosis protein including caspase-3, caspase-9 and Box, decrease the expression of autophagy-linked protein including Beclin-1 and LC3, increase anti-apoptosis protein Bcl-2, and restrain the activation of ERK and PPAR gamma. Conclusion: Cafestol preconditioning could attenuate inflammatory response, apoptosis and autophagy on hepatic ischemia reperfusion injury by suppressing ERK/PPAR gamma pathway.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81670472, 81700502, 81800538]; National Natural Science Foundation of Shanghai [:19ZR1447700]; Health System Innovation Plan of Shanghai Putuo District Science and Technology Committee [PTKWWS2018001]; WBN Liver Disease Research Fund of China Hepatitis Prevention Foundation [CFHPC2019031]; Yangfan Plan of Shanghai Science and Technology Commission [:2018YF1420000]; Project of Shanghai Health Commission [:2019469]
第一作者机构：[1]Tongji Univ, Dept Gastroenterol, Shanghai Peoples Hosp 10, Sch Med, Shanghai 200072, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Tongji Univ, Dept Gastroenterol, Shanghai Peoples Hosp 10, Sch Med, Shanghai 200072, Peoples R China[3]Tongji Univ, Putuo Peoples Hosp, Dept Gastroenterol, Sch Med, Shanghai 200060, Peoples R China
推荐引用方式(GB/T 7714)：
Ji Jie,Wu Liwei,Feng Jiao,et al.Cafestol preconditioning attenuates apoptosis and autophagy during hepatic ischemia-reperfusion injury by inhibiting ERK/PPAR gamma pathway[J].INTERNATIONAL IMMUNOPHARMACOLOGY.2020,84:doi:10.1016/j.intimp.2020.106529.
APA：
Ji, Jie,Wu, Liwei,Feng, Jiao,Mo, Wenhui,Wu, Jianye...&Guo, Chuanyong.(2020).Cafestol preconditioning attenuates apoptosis and autophagy during hepatic ischemia-reperfusion injury by inhibiting ERK/PPAR gamma pathway.INTERNATIONAL IMMUNOPHARMACOLOGY,84,
MLA：
Ji, Jie,et al."Cafestol preconditioning attenuates apoptosis and autophagy during hepatic ischemia-reperfusion injury by inhibiting ERK/PPAR gamma pathway".INTERNATIONAL IMMUNOPHARMACOLOGY 84.(2020)
