Objective: Hepatic ischemia reperfusion (IR) limits the development of liver transplantation technology. The aim of this study was to explore the protective effects of Bergenin on hepatic IR, particularly the elimination of reactive oxygen species (ROS) and activation of the peroxisome proliferators activated receptor gamma (PPAR-gamma) pathway. Methods: Initial experiments were performed to confirm the non-toxicity of Bergenin. Mice were randomly divided into sham, IR, and IR + Bergenin (10, 20 and 40 mg/kg) groups, and serum and tissue samples were obtained at 2, 8 and 24 h for detection of liver enzymes (ALT and AST), inflammatory factors (TNF-alpha, IL-6 and IL-1 beta), ROS, cell death markers (Bc1-2, Box, Beclin-1 and LC3) and related important pathways (PPAR-gamma, P38 MAPK, NF-kappa B p65 and JAK2/STAT1). Results: Bergenin reduced the release of ROS, down-regulated inflammatory factors, and inhibited apoptosis and autophagy. Additionally, expression of PPAR-gamma-related genes was increased and phosphorylation of P38 MAPK, NF-kappa B p65 and JAK2/STAT1-related proteins was decreased in Bergenin pre-treatment groups in a dose-dependent manner. Conclusion: Bergenin exerts hepatic protection by eliminating ROS, affecting the release of inflammatory factors, and influencing apoptosis- and autophagy-related genes via the PPAR-gamma pathway in this model of hepatic IR injury.
基金：
Science and Technology Committee of ShanghaiShanghai Science & Technology Committee [16411972700]; Science and Technology Committee of Changning District, Shanghai [CNKW2017Y05]
第一作者机构：[1]Soochow Univ, Med Coll, Suzhou 215006, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Soochow Univ, Med Coll, Suzhou 215006, Peoples R China[2]Tongji Univ, Dept Gastroenterol, Shanghai Peoples Hosp 10, Sch Med, Shanghai 200072, Peoples R China
推荐引用方式(GB/T 7714)：
Xiang Shihao,Chen Kan,Xu Ling,et al.Bergenin Exerts Hepatoprotective Effects by Inhibiting the Release of Inflammatory Factors, Apoptosis and Autophagy via the PPAR-gamma Pathway[J].DRUG DESIGN DEVELOPMENT AND THERAPY.2020,14:129-143.doi:10.2147/DDDT.S229063.
APA：
Xiang, Shihao,Chen, Kan,Xu, Ling,Wang, Ting&Guo, Chuanyong.(2020).Bergenin Exerts Hepatoprotective Effects by Inhibiting the Release of Inflammatory Factors, Apoptosis and Autophagy via the PPAR-gamma Pathway.DRUG DESIGN DEVELOPMENT AND THERAPY,14,
MLA：
Xiang, Shihao,et al."Bergenin Exerts Hepatoprotective Effects by Inhibiting the Release of Inflammatory Factors, Apoptosis and Autophagy via the PPAR-gamma Pathway".DRUG DESIGN DEVELOPMENT AND THERAPY 14.(2020):129-143
