Physiological and pathophysiological differences exist between the left and right kidneys; however, the molecular bases for these differences remain unknown. Since miRNAs are involved in kidney function and the development of kidney diseases, we examined their differential expression through miRNA profiling of the left and right kidneys of normal mice. We find that 36 miRNAs exhibit higher expression, whereas 22 miRNAs show lower expression in the left than the right kidneys in mice under physiological condition. Ten miRNAs were further examined by quantitative PCR assays, and four of them with high expression level were confirmed by Northern blot. Through bioinformatic analysis, we dissected the function and network of the differentially expressed miRNAs, providing insights into the physiological and pathophysiological differences between the left and the right kidneys.
基金：
National Key Research Program of China [2017YFC0909600, 2016YFC1304803]; Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81670462/81970440/81921001/81870551/81670748/81471035/81972966/91749107]; Beijing Natural Science FoundationBeijing Natural Science Foundation [7171006]; State Key Laboratory of Cardiovascular Disease Grant [2018fk-02]
第一作者机构：[1]Peking Univ, Ctr Noncoding RNA Med, MOE Key Lab Mol Cardiovasc Sci, Dept Physiol & Pathophysiol,Sch Basic Med Sci,Dep, Beijing 100191, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Peking Univ, Ctr Noncoding RNA Med, MOE Key Lab Mol Cardiovasc Sci, Dept Physiol & Pathophysiol,Sch Basic Med Sci,Dep, Beijing 100191, Peoples R China[3]Peking Univ, Ctr Basic Med Res, Inst Med Innovat & Res, Hosp 3, Beijing 100191, Peoples R China[*1]Center of Basic Medical Research, Institute of Medical Innovation and Research, Peking University Third Hospital, Beijing 100191, China[*2]Department of Biomedical Informatics, Department of Physiology and Pathophysiology, School of Basic Medical Sciences, MOE Key Laboratory of Molecular Cardiovascular Sciences, Center for Noncoding RNA Medicine, Peking University, Beijing 100191, China[*3]Department of Biomedical Informatics, Department of Physiology and Pathophysiology, School of Basic Medical Sciences, MOE Key Laboratory of Molecular Cardiovascular Sciences, Center for Noncoding RNA Medicine, Peking University, Beijing 100191, China
推荐引用方式(GB/T 7714)：
Gao Yuanxu,Yang Weili,Jin Ling,et al.Profiling and bioinformatic analysis reveal differential microRNA expression in the left and right kidneys in normal mice[J].FEBS LETTERS.2020,594(4):636-645.doi:10.1002/1873-3468.13644.
APA：
Gao, Yuanxu,Yang, Weili,Jin, Ling,Xue, Lixiang,Yang, Jichun&Cui, Qinghua.(2020).Profiling and bioinformatic analysis reveal differential microRNA expression in the left and right kidneys in normal mice.FEBS LETTERS,594,(4)
MLA：
Gao, Yuanxu,et al."Profiling and bioinformatic analysis reveal differential microRNA expression in the left and right kidneys in normal mice".FEBS LETTERS 594..4(2020):636-645
