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Adipose-derived stem cells modified by BDNF gene rescue erectile dysfunction after cavernous nerve injury

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收录情况: ◇ SCIE ◇ 统计源期刊 ◇ CSCD-C ◇ 卓越:梯队期刊

机构: [1]Wuhan Univ, Wuhan Hosp 3, Tongren Hosp, Dept Endocrinol, Wuhan, Hubei, Peoples R China [2]Huazhong Univ Sci & Technol, Tongji Med Coll, Cent Hosp Wuhan, Dept Hepatobiliary Surg, Wuhan, Hubei, Peoples R China [3]Huazhong Univ Sci & Technol, Tongji Med Coll, Cent Hosp Wuhan, Dept Urol, Wuhan, Hubei, Peoples R China
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关键词: adipose-derived stem cells brain-derived neurotrophic factor cavernous nerve injury erectile dysfunction infection intracavernous injection lentiviral vector neuronal nitric oxide synthase radical prostatectomy

摘要:
Cavernous nerve injury is the main cause of erectile dysfunction following radical prostatectomy. The recovery of erectile function following radical prostatectomy remains challenging. Our previous studies found that injecting adipose-derived stem cells (ADSCs) into the cavernosa could repair the damaged cavernous nerves, but the erectile function of the treated rats could not be restored to a normal level. We evaluated the efficacy of ADSCs infected with a lentiviral vector encoding rat brain-derived neurotrophic factor (lenti-rBDNF) in a rat model of cavernous nerve injury. The rats were equally and randomly divided into four groups. In the control group, bilateral cavernous nerves were isolated but not injured. In the bilateral cavernous nerve injury group, bilateral cavernous nerves were isolated and injured with a hemostat clamp for 2 minutes. In the ADSC(GFP) and ADSC(rBDNF) groups, after injury with a hemostat clamp for 2 minutes, rats were injected with ADSCs infected with lenti-GFP (1 x 10(6) in 20 mu L) and lenti-rBDNF (1 x 10(6) in 20 mu L), respectively. Erectile function was assessed 4 weeks after injury by measuring intracavernosal pressures. Then, penile tissues were collected for histological detection and western blot assay. Results demonstrated that compared with the bilateral cavernous nerve injury group, erectile function was significantly recovered in the ADSC(GFP) and ADSC(rBDNF) groups, and to a greater degree in the ADSC(rBDNF) group. Neuronal nitric oxide synthase content in the dorsal nerves and the ratio of smooth muscle/collagen were significantly higher in the ADSC(rBDNF) and ADSC(GFP) groups than in the bilateral cavernous nerve injury group. Neuronal nitric oxide synthase expression was obviously higher in the ADSC(rBDNF) group than in the ADSC(GFP) group. These findings confirm that intracavernous injection with ADSCs infected with lenti-rBDNF can effectively improve erectile dysfunction caused by cavernous nerve injury.

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出版当年[2019]版:
大类 | 3 区 医学
小类 | 3 区 细胞生物学 3 区 神经科学
最新[2023]版:
大类 | 2 区 医学
小类 | 3 区 细胞生物学 3 区 神经科学
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出版当年[2018]版:
Q3 NEUROSCIENCES Q3 CELL BIOLOGY
最新[2023]版:
Q1 NEUROSCIENCES Q2 CELL BIOLOGY

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第一作者机构: [1]Wuhan Univ, Wuhan Hosp 3, Tongren Hosp, Dept Endocrinol, Wuhan, Hubei, Peoples R China
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