Long noncoding RNA FOXD3-AS1 promotes colon adenocarcinoma progression and functions as a competing endogenous RNA to regulate SIRT1 by sponging miR-135a-5p
More and more documents have proved that the abnormal expression of long noncoding RNAs (lncRNAs) are correlated with the initiation and progression of colorectal cancer (CRC). lncRNA FOXD3-AS1 has been reported in glioma for its oncogenic property. According to the survival analysis of The Cancer Genome Atlas database, FOXD3-AS1 upregulation implied lower survival rate of patients with CRC. Quantitative real-time polymerase chain reaction showed the overexpression of FOXD3-AS1 in both CRC tissues and cells. The Kaplan-Meier method demonstrated the prognostic value of FOXD3-AS1 for patients with CRC. To explore the effect of FOXD3-AS1 on CRC progression, loss-of-function experiments were carried out, whose results indicated that knockdown of FOXD3-AS1 suppressed cell proliferation, migration, and invasion, inhibited cell cycle and promoted cell apoptosis in vitro. In vivo experiments affirmed that FOXD3-AS1 affected tumor growth. FOXD3-AS1 expression was enriched in the cytoplasm of CRC cells. Mechanism experiments revealed that FOXD3-AS1 served as a competing endogenous RNA to upregulate SIRT1 by sponging miR-135a-5p. In addition, SIRT1 silencing also restrained cell proliferation and motility. Rescue assays revealed the biological function of FOXD3-AS1/miR-135a-5p/SIRT1 axis in CRC progression. In conclusion, FOXD3-AS1 promotes CRC progression by regulating miR-135a-5p/SIRT1 axis, shedding lights on the way to CRC treatments.
基金：
Shanghai Municipal Health Bureau [20134100]; Research Fund of Shanghai Tongren Hospital, Shanghai Jiao Tong University School of Medicine [TRYJ201511, TRYJ201613]; Shanghai Municipal Health Bureau Key Disciplines Grant [ZK2015A24]; National Natural Science Foundation of the Science and Technology Commission of Shanghai Municipality [14ZR1431600, 1541197 1000, 14411973700]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81871912, 81570549, 81472242]
第一作者机构：[1]Shanghai Jiao Tong Univ, Tongren Hosp, Dept Gastroenterol, Sch Med, 1111 XianXia Rd, Shanghai 200336, Peoples R China
通讯作者：
通讯机构：[1]Shanghai Jiao Tong Univ, Tongren Hosp, Dept Gastroenterol, Sch Med, 1111 XianXia Rd, Shanghai 200336, Peoples R China[*1]Department of Gastroenterology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 XianXia Road, Shanghai 200336, China
推荐引用方式(GB/T 7714)：
Wu Qiong,Shi Min,Meng Wenying,et al.Long noncoding RNA FOXD3-AS1 promotes colon adenocarcinoma progression and functions as a competing endogenous RNA to regulate SIRT1 by sponging miR-135a-5p[J].JOURNAL OF CELLULAR PHYSIOLOGY.2019,234(12):21889-21902.doi:10.1002/jcp.28752.
APA：
Wu, Qiong,Shi, Min,Meng, Wenying,Wang, Yugang,Hui, Pingping&Ma, Jiali.(2019).Long noncoding RNA FOXD3-AS1 promotes colon adenocarcinoma progression and functions as a competing endogenous RNA to regulate SIRT1 by sponging miR-135a-5p.JOURNAL OF CELLULAR PHYSIOLOGY,234,(12)
MLA：
Wu, Qiong,et al."Long noncoding RNA FOXD3-AS1 promotes colon adenocarcinoma progression and functions as a competing endogenous RNA to regulate SIRT1 by sponging miR-135a-5p".JOURNAL OF CELLULAR PHYSIOLOGY 234..12(2019):21889-21902
