Here, we focused on the potential function of Gab3, an uncommon Gab family protein, in human colorectal cancer (CRC) cells. We found that Gab3 was only expressed in human colon cancer tissues as well as in established (HCT-116 and HT-29 lines) and primary human CRC cells. It was however absent in normal human colon cancer tissues and in FHC colon epithelial cells. Knockdown of Gab3 by targetedshRNAs inhibited proliferation of the CRC cells. Reversely, exogenous over-expression of Gab3 promoted CRC cell proliferation. At the signaling level, Gab3 co-precipitated with p85 and SHP2 in CRC cells, which was required for subsequent Akt and Erk activation. Gab3 shRNA knockdown inhibited Akt and Erk activation, yet Gab3 over-expression augmented it. In vivo, HCT-116 xenograft tumor growth in severe combined immune deficient (SCID) mice was suppressed following expressing Gab3 shRNAs. Meanwhile, Akt and Erk activation in Gab3 shRNA-expressing tumors was also largely inhibited. Together, our results suggest that Gab3 expression in CRC cells is important for Akt-Erk activation and cell proliferation. (C) 2017 Elsevier Inc. All rights reserved.
第一作者机构：[1]Shanghai Jiao Tong Univ, Sch Med, Shanghai Tongren Hosp, Dept Gastroenterol, 1111 Xianxia St, Shanghai 200336, Peoples R China[*1]Department of Gastroenterology, Shanghai Tongren Hospital, Affiliated to Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Street, Shanghai, 200336, China
共同第一作者：
通讯作者：
通讯机构：[1]Shanghai Jiao Tong Univ, Sch Med, Shanghai Tongren Hosp, Dept Gastroenterol, 1111 Xianxia St, Shanghai 200336, Peoples R China[*1]Department of Gastroenterology, Shanghai Tongren Hospital, Affiliated to Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Street, Shanghai, 200336, China
推荐引用方式(GB/T 7714)：
Xiang Shihao,Wang Na,Hui Pingping,et al.Gab3 is required for human colorectal cancer cell proliferation[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2017,484(4):719-725.doi:10.1016/j.bbrc.2017.01.095.
APA：
Xiang, Shihao,Wang, Na,Hui, Pingping&Ma, Jiali.(2017).Gab3 is required for human colorectal cancer cell proliferation.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,484,(4)
MLA：
Xiang, Shihao,et al."Gab3 is required for human colorectal cancer cell proliferation".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 484..4(2017):719-725
