机构：[1]Department of Obstetrics and Gynecology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China[2]Department of Interventional & Vascular Surgery, Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China[3]Department of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China[4]Department of Endocrinology, The First Affiliated Hospital of Fujian Medical University, Diabetes Research Insititute of Fujian Province, Fuzhou, Fujian, China[5]Department of Obstetrics and Gynecology, Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China
Increasing evidence demonstrates the crucial regulatory functions of circular RNAs in different cancer types. The major aim of the current study was to establish functions of circPIP5K1A during ovarian cancer. Our results showed an increased expression of circPIP5K1A in both ovarian cancers and cell lines, which was associated with poor prognosis. In functional analyses, downregulation of circPIP5K1A suppressed ovarian cancer cell migration, proliferation, and invasion in vitro. The miR-661 was indicated as a target of circPIP5K1A and insulin-like growth factor-binding protein 5 (IGFBP5) as a target of miR-661. circPIP5K1A silencing triggered downregulation of IGFBP5 through inducing an increase in miR-66 levels, as determined by the luciferase reporter assay. Data from cell counting kit-8, colony formation, wound healing, and Transwell assays showed that overexpression of IGFBP5 effectively reversed the circPIP5K1A depletion effects. The results collectively indicated that circPIP5K1A contributed to ovarian cancer progression via targeting the miR-661/IGFBP5 axis, supporting its utility as a candidate target for therapy of the disease.
基金：
Natural Science Foundation of Fujian
Province of China, Grant/Award Number:
2016J01542
第一作者机构：[1]Department of Obstetrics and Gynecology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Obstetrics and Gynecology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China[3]Department of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China[4]Department of Endocrinology, The First Affiliated Hospital of Fujian Medical University, Diabetes Research Insititute of Fujian Province, Fuzhou, Fujian, China[*1]Department of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, No. 507, Zhengmin Road, Yangpu District, 200433 Shanghai, China.[*2]Department of Endocrinology, The First Affiliated Hospital of Fujian Medical University, Diabetes Research Insititute of Fujian Province, No. 20, Chazhong road, Taijiang District, Fuzhou, 350005 Fujian, China[*3]Department of Obstetrics and Gynecology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, 1111 Xianxia Road, 200336 Shanghai, China.
推荐引用方式(GB/T 7714)：
Sun Yi,Lie Xue,Chen Aozheng,et al.circPIP5K1A serves as a competitive endogenous RNA contributing to ovarian cancer progression via regulation of miR-661/IGFBP5 signaling[J].JOURNAL OF CELLULAR BIOCHEMISTRY.2019,120(12):19406-19414.doi:10.1002/jcb.29055.
APA：
Sun, Yi,Lie, Xue,Chen, Aozheng,Shi, Weihui,Wang, Lei...&Qiu, Jin.(2019).circPIP5K1A serves as a competitive endogenous RNA contributing to ovarian cancer progression via regulation of miR-661/IGFBP5 signaling.JOURNAL OF CELLULAR BIOCHEMISTRY,120,(12)
MLA：
Sun, Yi,et al."circPIP5K1A serves as a competitive endogenous RNA contributing to ovarian cancer progression via regulation of miR-661/IGFBP5 signaling".JOURNAL OF CELLULAR BIOCHEMISTRY 120..12(2019):19406-19414
