Objective To compare genome-wide DNA methylation between samples of sinonasal inverted papilloma (SNIP) and squamous cell carcinoma (SCC) samples in order to identify aberrantly methylated genes that might be involved in malignant transformation. Methods Tissue samples were collected from patients. DNA methylation in C-phosphate-G islands and gene promoters was analysed using a DNA methylation microarray kit. The levels of mRNA or protein from aberrantly methylated genes were measured using real-time polymerase chain reaction or Western blot analysis. Results A total of 27 tissue samples were included in this study; 15 SNIP samples and 12 SCCs arising in SNIPs. A total of 11 201 nominally differentially methylated sites were observed between SNIP and SCC arising in SNIPs. Six sites were significantly different at P < 0.01 and contained three genes (MIR661, PLEC and OPA3). These three genes were hypermethylated. In addition, the levels of mature miR-661 mRNA and PLEC protein were significantly upregulated in SCC tissues compared with SNIP samples. The levels of OPA3 protein were downregulated in SCC tissues compared with SNIP samples. Conclusions This study demonstrated hypermethylation and abnormal expression of the MIR661, PLEC and OPA3 genes, suggesting a role for their involvement in the malignant transformation of SNIP.
基金:
Beijing Municipal Administration of Hospitals
Clinical Medicine Development of Special
Funding Support (no. XMLX201507), the
National Natural Science Foundation of China
(no. 81670946), the Beijing Key Laboratory of
Nasal Diseases, Capital Development Fund
for Medical Research (no. 2011-2005-06), the
Priming Scientific Research Foundation for
Senior Researchers in Beijing Tongren Hospital,
Capital Medical University (no. 2016-YJJ-GGL-
001) and 2018-1-2052 Capital’s Funds for Health
Improvement and Research (CFH2018-1-2052).
语种:
外文
被引次数:
WOS:
PubmedID:
中科院(CAS)分区:
出版当年[2018]版:
大类|4 区医学
小类|4 区医学:研究与实验4 区药学
最新[2023]版:
大类|4 区医学
小类|4 区医学:研究与实验4 区药学
JCR分区:
出版当年[2017]版:
Q4PHARMACOLOGY & PHARMACYQ4MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版:
Q4MEDICINE, RESEARCH & EXPERIMENTALQ4PHARMACOLOGY & PHARMACY
第一作者机构:[1]Capital Med Univ, Beijing Tongren Hosp, Dept Otolaryngol Head & Neck Surg, 1 Dong Jiao Min Xiang St, Beijing 100730, Peoples R China[2]Minist Educ, Key Lab Otolaryngol Head & Neck Surg, Beijing, Peoples R China
通讯作者:
通讯机构:[1]Capital Med Univ, Beijing Tongren Hosp, Dept Otolaryngol Head & Neck Surg, 1 Dong Jiao Min Xiang St, Beijing 100730, Peoples R China[2]Minist Educ, Key Lab Otolaryngol Head & Neck Surg, Beijing, Peoples R China[*1]Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 1 Dong Jiao Min Xiang Street, DongCheng District, Beijing 100730, China
推荐引用方式(GB/T 7714):
Yang Zheng,Zhang Yang,Wang Xiangdong,et al.Putative biomarkers of malignant transformation of sinonasal inverted papilloma into squamous cell carcinoma[J].JOURNAL OF INTERNATIONAL MEDICAL RESEARCH.2019,47(6):2371-2380.doi:10.1177/0300060519838385.
APA:
Yang, Zheng,Zhang, Yang,Wang, Xiangdong,Huang, Junwei,Guo, Wei...&Zhang, Luo.(2019).Putative biomarkers of malignant transformation of sinonasal inverted papilloma into squamous cell carcinoma.JOURNAL OF INTERNATIONAL MEDICAL RESEARCH,47,(6)
MLA:
Yang, Zheng,et al."Putative biomarkers of malignant transformation of sinonasal inverted papilloma into squamous cell carcinoma".JOURNAL OF INTERNATIONAL MEDICAL RESEARCH 47..6(2019):2371-2380