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Putative biomarkers of malignant transformation of sinonasal inverted papilloma into squamous cell carcinoma

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机构: [1]Capital Med Univ, Beijing Tongren Hosp, Dept Otolaryngol Head & Neck Surg, 1 Dong Jiao Min Xiang St, Beijing 100730, Peoples R China [2]Minist Educ, Key Lab Otolaryngol Head & Neck Surg, Beijing, Peoples R China [3]Univ Texas MD Anderson Canc Ctr, Dept Biostat, Houston, TX 77030 USA [4]Univ Texas MD Anderson Canc Ctr, Dept Head & Neck Surg, Houston, TX 77030 USA [5]Univ Texas MD Anderson Canc Ctr, Dept Epidemiol, Houston, TX 77030 USA
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关键词: Sinonasal inverted papilloma squamous cell carcinoma methylation C-phosphate-G islands epigenetics

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Objective To compare genome-wide DNA methylation between samples of sinonasal inverted papilloma (SNIP) and squamous cell carcinoma (SCC) samples in order to identify aberrantly methylated genes that might be involved in malignant transformation. Methods Tissue samples were collected from patients. DNA methylation in C-phosphate-G islands and gene promoters was analysed using a DNA methylation microarray kit. The levels of mRNA or protein from aberrantly methylated genes were measured using real-time polymerase chain reaction or Western blot analysis. Results A total of 27 tissue samples were included in this study; 15 SNIP samples and 12 SCCs arising in SNIPs. A total of 11 201 nominally differentially methylated sites were observed between SNIP and SCC arising in SNIPs. Six sites were significantly different at P < 0.01 and contained three genes (MIR661, PLEC and OPA3). These three genes were hypermethylated. In addition, the levels of mature miR-661 mRNA and PLEC protein were significantly upregulated in SCC tissues compared with SNIP samples. The levels of OPA3 protein were downregulated in SCC tissues compared with SNIP samples. Conclusions This study demonstrated hypermethylation and abnormal expression of the MIR661, PLEC and OPA3 genes, suggesting a role for their involvement in the malignant transformation of SNIP.

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出版当年[2018]版:
大类 | 4 区 医学
小类 | 4 区 医学:研究与实验 4 区 药学
最新[2023]版:
大类 | 4 区 医学
小类 | 4 区 医学:研究与实验 4 区 药学
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出版当年[2017]版:
Q4 PHARMACOLOGY & PHARMACY Q4 MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版:
Q4 MEDICINE, RESEARCH & EXPERIMENTAL Q4 PHARMACOLOGY & PHARMACY

影响因子: 最新[2023版] 最新五年平均 出版当年[2017版] 出版当年五年平均 出版前一年[2016版] 出版后一年[2018版]

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第一作者机构: [1]Capital Med Univ, Beijing Tongren Hosp, Dept Otolaryngol Head & Neck Surg, 1 Dong Jiao Min Xiang St, Beijing 100730, Peoples R China [2]Minist Educ, Key Lab Otolaryngol Head & Neck Surg, Beijing, Peoples R China
通讯作者:
通讯机构: [1]Capital Med Univ, Beijing Tongren Hosp, Dept Otolaryngol Head & Neck Surg, 1 Dong Jiao Min Xiang St, Beijing 100730, Peoples R China [2]Minist Educ, Key Lab Otolaryngol Head & Neck Surg, Beijing, Peoples R China [*1]Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 1 Dong Jiao Min Xiang Street, DongCheng District, Beijing 100730, China
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