机构：[1]Department of Laboratory Medicine, Beijing Tongren Hospital, Capital Medical University, Beijing, China医技科室检验科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]College of Medicine, Capital Medical University, Beijing, China[3]Ningbo HEALTH Gene Technologies Co., Ltd., Ningbo, China[4]Department of Laboratory Medicine, Beijing Luhe Hospital, Capital Medical University, Beijing, China
Community-acquired pneumonia (CAP) is a common infectious disease linked to high rates of morbidity and mortality. Fast and accurate identification of the pathogens responsible for CAP will aid in diagnosis. We established a capillary electrophoresis-based multiplex PCR (CEMP) panel to enable the detection of viral and bacterial pathogens associated with CAP. The assay simultaneously detects and identifies the 13 common unculturable CAP viral and bacterial pathogens within 4 h. We evaluated the performance of a commercially available panel with 314 samples collected from CAP patients. We compared the results to those obtained with the liquid chip-based Luminex xTAG Respiratory Viral Panel (RVP) Fast Kit (for viruses) and the agarose gel-based Seegene PneumoBacter ACE Detection Kit (for atypical bacteria). All positive samples were further verified by the Sanger sequencing method. The sensitivity, specificity, positive predictive value, and negative predictive value of CEMP were 97.31%, 100%, 100%, and 99.85%, respectively. CEMP provides a rapid and accurate method for the high-throughput detection of pathogens in patients with CAP.
基金：
Ministry of Science and Technology of the People's Republic of ChinaMinistry of Science and Technology, China [2014ZX10004005]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区工程技术
小类|3 区分析化学4 区生化研究方法
最新[2023]版：
大类|4 区医学
小类|4 区生化研究方法4 区分析化学
JCR分区：
出版当年[2017]版：
Q4BIOCHEMICAL RESEARCH METHODSQ4CHEMISTRY, ANALYTICAL
最新[2023]版：
Q3BIOCHEMICAL RESEARCH METHODSQ3CHEMISTRY, ANALYTICAL
第一作者机构：[1]Department of Laboratory Medicine, Beijing Tongren Hospital, Capital Medical University, Beijing, China[2]College of Medicine, Capital Medical University, Beijing, China
通讯作者：
通讯机构：[1]Department of Laboratory Medicine, Beijing Tongren Hospital, Capital Medical University, Beijing, China[2]College of Medicine, Capital Medical University, Beijing, China[*1]Department of Laboratory Medicine, Beijing Tongren Hospital, Capital Medical University, No. 1 Dongjiaominxiang St., Dongcheng District, Beijing 100730, China.
推荐引用方式(GB/T 7714)：
Li Xue,Chen Bo,Zhang Shaoya,et al.Rapid Detection of Respiratory Pathogens for Community-Acquired Pneumonia by Capillary Electrophoresis-Based Multiplex PCR[J].SLAS TECHNOLOGY.2019,24(1):105-116.doi:10.1177/2472630318787452.
APA：
Li, Xue,Chen, Bo,Zhang, Shaoya,Li, Xiuyuan,Chang, Junxia...&Lu, Xinxin.(2019).Rapid Detection of Respiratory Pathogens for Community-Acquired Pneumonia by Capillary Electrophoresis-Based Multiplex PCR.SLAS TECHNOLOGY,24,(1)
MLA：
Li, Xue,et al."Rapid Detection of Respiratory Pathogens for Community-Acquired Pneumonia by Capillary Electrophoresis-Based Multiplex PCR".SLAS TECHNOLOGY 24..1(2019):105-116
