详情页 - 首都医科大学附属北京同仁医院知识库

当前位置：首页 > 详情页

Diagnostic efficacy of targeted high-throughput sequencing for lower respiratory infection in preterm infants

1| 认领 | 导出 |

文献详情

资源类型：

WOS体系：

Pubmed体系：

收录情况： ◇ SCIE

作者：

机构： [1]Department of Neonatology, Children's Hospital of Fudan University, National Children's Medical Center Shanghai, China. [2]Geriatrics Department, Tong Ren Hospital, Shanghai Jiao Tong University School of Medicine Shanghai, China.

出处：

ISSN：

关键词： Targeted next-generation sequencing multiplex PCR respiratory infectious disease preterm infant pathogen detection diagnosis

摘要：

To examine the pathogen diagnostic performance of targeted high-throughput next-gen sequencing (tNGS) in respiratory infectious diseases in preterm infants using dynamic follow-up.Clinical samples of respiratory secretions were consecutively collected from 20 preterm infants weekly for 5 weeks, during which 10 developed bronchopulmonary dysplasia. Pathogen identification from these collected specimens was performed by both conventional cultivation and tNGS.We found that targeted next-generation sequencing shared a 90.9% full or partial consistency for lower respiratory pathogen detection with the traditional culture-based approach, and increased the detection rate by 105.9%. Moreover, most of the pathogens identified by tNGS were diminished in patients after treatment.This study reveals the high sensitivity and performance of targeted high-throughput sequencing for respiratory infectious disease diagnosis and pathogen identification. The trial registry number is NCT03850457, and the trial URL is https://clinicaltrials.gov/ct2/show/NCT03850457.AJTR Copyright © 2022.

语种：

被引次数：

WOS：

PubmedID：

中科院(CAS)分区：

出版当年[2021]版：

大类 | 3 区医学

小类 | 4 区肿瘤学 4 区医学：研究与实验

最新[2023]版：

大类 | 4 区医学

小类 | 4 区医学：研究与实验 4 区肿瘤学

JCR分区：

出版当年[2020]版：

Q2 MEDICINE, RESEARCH & EXPERIMENTAL Q3 ONCOLOGY

最新[2023]版：

Q3 MEDICINE, RESEARCH & EXPERIMENTAL Q4 ONCOLOGY

影响因子： 1.7 最新[2023版] 2.3 最新五年平均 4.06 出版当年[2020版] 4.011 出版当年五年平均 3.375 出版前一年[2019版] 3.94 出版后一年[2021版]

第一作者：

第一作者机构： [1]Department of Neonatology, Children's Hospital of Fudan University, National Children's Medical Center Shanghai, China.

共同第一作者：

通讯作者：

通讯机构： [1]Department of Neonatology, Children's Hospital of Fudan University, National Children's Medical Center Shanghai, China. [*1]Department of Neonatology, Children’s Hospital of Fudan University, National Children’s Medical Center, No. 399 Wanyuan Road, Shanghai, China

推荐引用方式(GB/T 7714)：

APA：

MLA：

相关文献

[1]靶向基因高通量测序技术诊断儿童基因组病的可行性研究 [2]Applications of targeted gene capture and next-generation sequencing technologies in studies of human deafness and other genetic disabilities [3]Application of the second-generation sequencing technology of metagenomics in the detection of pathogens in respiratory patients [4]Rapid Detection of Respiratory Pathogens for Community-Acquired Pneumonia by Capillary Electrophoresis-Based Multiplex PCR [5]Development of a multiplex real-time PCR assay for the simultaneous detection of mpox virus and orthopoxvirus infections [6]xtag?gpp多重核酸技术在北京地区感染性腹泻诊断中的应用及流行病学研究 [7]多重pcr同时检测病原菌及其耐药基因 [8]多重PCR检测方法和液态芯片技术在呼吸道病毒检测中的应用研究 [9]Correlation between apelin and VEGF levels in retinopathy of prematurity: a matched case-control study [10]Consensus on the diagnosis and treatment of blepharoptosis