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Development of a multiplex real-time PCR assay for the simultaneous detection of mpox virus and orthopoxvirus infections

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机构： [1]NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, PR China [2]NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China [3]Department of Medical Oncology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 100730, PR China [4]Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, PR China [5]Lady Davis Institute, Jewish General Hospital, McGill University, Montreal, Quebec H3T 1E2, Canada

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关键词： Mpox Orthopoxvirus Real-time PCR Virus detection

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Since May 2022, the multi-country outbreak of monkeypox (mpox) has raised a great concern worldwide. Early detection of mpox virus infection is recognized as an efficient way to prevent mpox transmission. Mpox specific detection methods reported up to now are based on the SNPs among mpox virus and other orthopoxviruses. We have therefore developed a real-time PCR based mpox detection method targeting mpox virus specific sequences (N3R and B18Rplus). We have also optimized an orthopoxvirus detection system which targets the highly conserved E9L and D6R genes. The mpox and orthopoxvirus real-time PCR assays have a high sensitivity (1 copy/reaction) and specificity. Mpox viral DNA and clinical samples from mpox patients are detected with the mpox detection system. Furthermore, we have established a multiplex real-time PCR detection system allowing simultaneous and efficient detection of mpox and orthopoxvirus infections.Copyright © 2024. Published by Elsevier B.V.

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出版当年[2023]版：

大类 | 4 区医学

小类 | 3 区生化研究方法 3 区生物工程与应用微生物 4 区病毒学

最新[2023]版：

大类 | 4 区医学

小类 | 3 区生化研究方法 3 区生物工程与应用微生物 4 区病毒学

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出版当年[2022]版：

Q2 BIOCHEMICAL RESEARCH METHODS Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Q3 VIROLOGY

最新[2023]版：

Q3 BIOCHEMICAL RESEARCH METHODS Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Q3 VIROLOGY

影响因子： 2.2 最新[2023版] 2 最新五年平均 3.1 出版当年[2022版] 2.4 出版当年五年平均 2.623 出版前一年[2021版] 2.2 出版后一年[2023版]

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第一作者机构： [1]NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, PR China

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