Combination of TPF regimen and cinobufotalin inhibits proliferation and induces apoptosis in human hypopharyngeal and laryngeal squamous cell carcinoma cells
Background: Hypopharyngeal squamous cell carcinoma and laryngeal squamous cell carcinoma have a potential risk of metastasis and local recurrence. It is urgent to determine novel effective therapy. Methods: UMSCC5 and FADU cells were treated with TPF regimen (a mixture comprising paclitaxel, cisplatin, and 5-fluorouracil), cinobufotalin, and combination. Cell counting kit-8 (CCK-8), clone formation assays, and flow cytometry were performed to investigate whether combination of cinobufotalin and TPF can exhibit synergistic effects in reducing tumor growth. Results: CCK-8 assay and IC50 analysis showed that the TPF and cinobufotalin could suppress viability of UMSCC5 and FADU cells, implying the strong antitumor effect. The synergism between TPF and cinobufotalin was further verified by the CCK-8 and clone formation assays showing the TPF- and cinobufotalin-suppressed cell proliferation synergistically. Notably, flow cytometry showed that the combination also promoted apoptosis synergistically. Conclusion: TPF regimen combining cinobufotalin suppressed proliferation and promoted apoptosis synergistically in hypopharyngeal squamous cell carcinoma and laryngeal squamous cell carcinoma cells.
基金：
Beijing Municipal Administration of Hospitals' Ascent Plan [DFL20180202]; Beijing Municipal Administration of Hospitals Incubating Program [PX2018009]; Beijing Administration of Traditional Chinese Medicine [QN2018-32]
第一作者机构：[1]Capital Med Univ, Dept Otorhinolaryngol Head & Neck Surg, Beijing Tongren Hosp, 1 Dongjiaominxiang St, Beijing 100730, Peoples R China
通讯作者：
通讯机构：[1]Capital Med Univ, Dept Otorhinolaryngol Head & Neck Surg, Beijing Tongren Hosp, 1 Dongjiaominxiang St, Beijing 100730, Peoples R China[*1]Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 1st Dongjiaominxiang Street, Dongcheng District, Beijing 100730, China
推荐引用方式(GB/T 7714)：
Shen Xixi,Tao Yong,Yang Yifan,et al.Combination of TPF regimen and cinobufotalin inhibits proliferation and induces apoptosis in human hypopharyngeal and laryngeal squamous cell carcinoma cells[J].ONCOTARGETS AND THERAPY.2019,12:341-348.doi:10.2147/OTT.S185428.
APA：
Shen, Xixi,Tao, Yong,Yang, Yifan,Wang, Ru,Fang, Jugao&Lian, Meng.(2019).Combination of TPF regimen and cinobufotalin inhibits proliferation and induces apoptosis in human hypopharyngeal and laryngeal squamous cell carcinoma cells.ONCOTARGETS AND THERAPY,12,
MLA：
Shen, Xixi,et al."Combination of TPF regimen and cinobufotalin inhibits proliferation and induces apoptosis in human hypopharyngeal and laryngeal squamous cell carcinoma cells".ONCOTARGETS AND THERAPY 12.(2019):341-348
