机构：[1]Department of Invasive Technology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai 200336, China.
A role of microRNA-4262 (miR-4262) in the carcinogenesis of colon cancer remains undetermined. In this study, we studied the effects and mechanisms of miR-4262 to the colon cancer cell proliferation and apoptosis. We found that the levels of miR-4262 significantly down-regulated in colon cancer tissue, compared to the paired adjacent non-tumor colon tissue. The miR-4262 levels in colon cancer cell lines were significantly lower than those in control normal colon tissues. Transfection with the miR-4262 mimic decreased the cell proliferation and increased cell apoptosis in colon cancer cells, while transfection with the antisense of miR-4262 (as-miR-4262) increased cell proliferation and suppressed cell apoptosis in colon cancer cells. Bioinformatics analyses showed that GALNT4 was a potential target gene of miR-4262. The luciferase activities assay and Western blot verified that miR-4262 targeted GALNT4 mRNA to modulate its protein levels. When we treated cells with miR-4262 and GALN4 siRNA, the cell viability was significantly decreased. Together, our study suggests that aberrantly expressed miR-4262 may affect cell apoptosis and proliferation of human colon cancer cells via GALNT4, which appears to be a promising therapeutic target for colon cancer.
基金：
The work was supported by the Tongren Hos-pital Medical Research Funding Program and the Key Medical Specialty Project of Shanghai (ZK2015A26).
第一作者机构：[1]Department of Invasive Technology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai 200336, China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Invasive Technology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai 200336, China.[*1]Department of Invasive Technology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai 200336, China.
推荐引用方式(GB/T 7714)：
Weng Li,Ma Jun,Jia Yi-Ping,et al.MiR-4262 promotes cell apoptosis and inhibits proliferation of colon cancer cells: involvement of GALNT4[J].AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH.2018,10(12):3969-3977.
APA：
Weng, Li,Ma, Jun,Jia, Yi-Ping,Wu, Shao-Qiu,Liu, Bin-Yan...&Mao, Ai-Wu.(2018).MiR-4262 promotes cell apoptosis and inhibits proliferation of colon cancer cells: involvement of GALNT4.AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH,10,(12)
MLA：
Weng, Li,et al."MiR-4262 promotes cell apoptosis and inhibits proliferation of colon cancer cells: involvement of GALNT4".AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH 10..12(2018):3969-3977
