Dexamethasone (Dex) can induce injury to human osteoblasts. Long non-coding RNA (LncRNA) EPIC1 (Lnc-EPIC1) is a novel Myc-interacting LncRNA. Its effect on Dex-treated human osteoblasts is studied here. In OB-6 osteoblastic cells and primary human osteoblasts, treatment with Dex increased expression of Lnc-EPIC1. Its expression is also elevated in the necrotic femoral head tissues of Dex-taking patients. Ectopic overexpression of Lnc-EPIC1 inhibited Dex-induced apoptosis and programmed necrosis in OB-6 cells and primary human osteoblasts. Reversely, Lnc-EPIC1 silencing by targeted siRNA potentiated Dex-induced cytotoxicity. Myc is the target of Lnc-EPIC1 in osteoblasts. Exogenous overexpression of Myc protected OB-6 cells from Dex. Conversely, Myc knockout by CRISPR-Cas-9 method abolished Lnc-EPIC1-induced OB-6 cytoprotection against Dex. Together, Lnc-EPIC1 expression protects human osteoblasts from Dex possible via regulation of Myc. (C) 2018 Elsevier Inc. All rights reserved.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81171712]; Natural Science Foundation of Jiangsu ProvinceNatural Science Foundation of Jiangsu Province [BK20151213]; Innovation Project of Jiangsu Province [BK 201423, BK20160340]
第一作者机构：[1]Soochow Univ, Affiliated Hosp 2, Dept Orthoped, Suzhou, Peoples R China[2]Shanghai Jiao Tong Univ, Tongren Hosp, Dept Orthoped, Sch Med, Shanghai, Peoples R China
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推荐引用方式(GB/T 7714)：
Zhang Xiang-yang,Shan Hua-jian,Zhang Peng,et al.LncRNA EPIC1 protects human osteoblasts from dexamethasone-induced cell death[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2018,503(4):2255-2262.doi:10.1016/j.bbrc.2018.06.146.
APA：
Zhang, Xiang-yang,Shan, Hua-jian,Zhang, Peng,She, Chang&Zhou, Xiao-zhong.(2018).LncRNA EPIC1 protects human osteoblasts from dexamethasone-induced cell death.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,503,(4)
MLA：
Zhang, Xiang-yang,et al."LncRNA EPIC1 protects human osteoblasts from dexamethasone-induced cell death".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 503..4(2018):2255-2262
