机构：[1]Department of Clinical Laboratory, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.医技科室检验科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.[3]Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China.[4]Beijing Key Laboratory of Emerging Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.[5]Surgical Intensive Care Unit, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.首都医科大学附属北京同仁医院首都医科大学附属同仁医院
Background: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a major cause of nosocomial infections worldwide. The transmission route of CRKP isolates within an outbreak is rarely described. This study aimed to reveal the molecular characteristics and transmission route of CRKP isolates within an outbreak of nosocomial infection. Methods: Collecting case information, active screening and targeted environmental monitoring were carried out. The antibiotic susceptibility, drug-resistant genes, molecular subtype and whole genome sequence of CRKP strains were analyzed. Results: Between October and December 2011, 26 CRKP isolates were collected from eight patients in a surgical intensive care unit and subsequent transfer wards of Beijing Tongren hospital, China. All 26 isolates harbored bla(KPC-2), bla(SHV-1), and bla(CTX-M-15) genes, had the same or similar pulsed-field gel electrophoresis patterns, and belonged to the sequence type 11 (ST11) clone. By comprehensive consideration of genomic and epidemiological information, a putative transmission map was constructed, including identifying one case as an independent event distinct from the other seven cases, and revealing two transmissions starting from the same case. Conclusions: This study provided the first report confirming an outbreak caused by K pneumoniae ST11 clone co-harboring the bla(KPC-2), bla(CTX-M-15), and bla(SHV-1) genes, and suggested that comprehensive consideration of genomic and epidemiological data can yield a fine transmission map of an outbreak and facilitate the control of nosocomial transmission.
基金：
This work was supported by grants from the National Natural Science
Foundation of China (NSFC) [grant number 81371861], the Priority Project on
Infectious Disease Control and Prevention [grant number 2012ZX10004215]
from the Ministry of Science and Technology of the People’s Republic of
China and the Capacity-building project for pathogenic bacteria monitoring
from Health and Family Planning Commission of the People’s Republic of
China [grant number 131031102000150003].
第一作者机构：[1]Department of Clinical Laboratory, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
共同第一作者：
通讯作者：
通讯机构：[2]State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.[3]Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China.
推荐引用方式(GB/T 7714)：
Sui Wenjun,Zhou Haijian,Du Pengcheng,et al.Whole genome sequence revealed the fine transmission map of carbapenem-resistant Klebsiella pneumonia isolates within a nosocomial outbreak[J].ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL.2018,7:doi:10.1186/s13756-018-0363-8.
APA：
Sui, Wenjun,Zhou, Haijian,Du, Pengcheng,Wang, Lijun,Qin, Tian...&Lu, Xinxin.(2018).Whole genome sequence revealed the fine transmission map of carbapenem-resistant Klebsiella pneumonia isolates within a nosocomial outbreak.ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL,7,
MLA：
Sui, Wenjun,et al."Whole genome sequence revealed the fine transmission map of carbapenem-resistant Klebsiella pneumonia isolates within a nosocomial outbreak".ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL 7.(2018)
