Treatment of ventricular infection is challenging. Parenteral antibiotic therapy has limited efficacy owing to the blood brain barrier. Other treatment options like intraventricular irrigation and intrathecal injection of antibiotics have a limited role owing to complications. In this study we investigate a novel minimally invasive neuroendoscopic approach for treatment of infection of ventricular system. A total of fourteen patients (9 male and 5 female) with ventricular infection were enrolled in the study. All patients were assessed and treated via neuroendoscope. The infection severity was evaluated, followed by removal of the intraventricular sediment and mural pus. Through the help of reserved bilateral drainage tubes, postoperative irrigation with antibiotic saline was carried out for a period of two to four weeks. Additionally, in patients with hydrocephalus, ventriculoperitoneal (VP) shunt operation or endoscopic third ventriculostomy (ETV) was also performed. One patient died of respiratory failure, and the remaining thirteen patients were cured with a mean Glasgow outcome scale (GOS) grade of 2.8 +/- 0.9. Among the nine patients with hydrocephalus, five underwent VP shunt, three received ETV, and one underwent VP shunt after a failed attempt at ETV treatment. The duration of follow-up period ranged from 5 to 22 months (median 12.5 months), and no recurrence of ventricular infection was reported. Our results suggest the potential of neuroendoscope as a valuable tool for assessment and treatment of ventricular infection. Postoperative continuous irrigation with antibiotic saline appears to have a key role in achieving favorable outcomes using this method.