Background: Laryngeal squamous cell carcinoma (LSCC) is the most common type of head and neck squamous cell carcinoma (HNSCC), and comprehensive post-transcriptional regulation is needed to be clarified. Objective: The purpose of this study was to investigate the miRNA basis of tumorigenesis in LSCC, and miRNA-gene-network and miRNA-pathway-network will be established with microarray analysis to reveal the molecular mechanism regulated by miRNAs. Methods: MicroRNA array and microarray were used to detect differently expressed miRNAs and mRNAs between the LSCC samples and para-carcinoma tissues collected from 2008 to 2013, then miRNA-mRNA network and miRNA-pathway network analysis were performed. Finally, the top dysregulated miRNAs were validated by quantitative real-time polymerase chain reaction (qRT-PCR). Results: 50 miRNAs (47 up-and 3 down-regulated), 2381 mRNAs (1542 up-and 839 down-regulated) were found to be differentially expressed. Bioinformatics analysis show that there were 31 different genes and 71 functional pathways regulated by the screened miRNAs. Hsa-miR-30a-5p expression level was validated by qRT-PCR. Conclusions: The research revealed tumorigenesis-related miRNAs, mRNAs, miRNA-mRNA, miRNA-pathway-network in LSCC. Hsa-miR-30a-5p could be considered as a potential diagnostic and therapeutic marker in LSCC.
基金：
Inner Mongolia Autonomous Region Natural Science Foundation [2015MS0834]; Beijing Municipal Science & Technology CommissionBeijing Municipal Science & Technology Commission [Z141107002514003]; Beijing Municipal Administration of Hospital Clinical Medicine Development of Special Funding Support [XMLX201311]; The Priming Scientific Research Foundation for The Junior Researcher in Beijing Tongren Hospital, Capital Medical University [2015-YJJ-ZZL-001]
第一作者机构：[1]Capital Med Univ, Beijing Tongren Hosp, Dept Otorhinolaryngol Head & Neck Surg, 2 Chongwen Mennei St, Beijing 100730, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Tongren Hosp, Dept Otorhinolaryngol Head & Neck Surg, 2 Chongwen Mennei St, Beijing 100730, Peoples R China[2]Inner Mongolia Autonomous Reg Peoples Hosp, Dept Otolaryngol, 20 Zhaowuda St, Hohhot 010010, Inner Mongolia, Peoples R China[*1]Depart-ment of Otolaryngology, The Inner Mongolia Auto-nomous Region People’s Hospital, No.20, Zhaowu-da Street, Saihan District, Huhhot, Inner Mongolia 010010, PR China[*2]Department of Otorhinolaryngo-logy-Head and Neck Surgery, Beijing Tongren Hos-pital, Capital Medical University, No.2, Chongwen-mennei Street, Dongcheng District, Beijing 100730, PR China.
推荐引用方式(GB/T 7714)：
Yang Yifan,Lian Meng,Feng Ling,et al.Array profiling identified dysregulated miRNAs and target genes and pathways in laryngeal squamous cell carcinoma[J].INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE.2018,11(3):1973-1983.
APA：
Yang, Yifan,Lian, Meng,Feng, Ling,Wang, Ru,Shi, Qian...&Fang, Jugao.(2018).Array profiling identified dysregulated miRNAs and target genes and pathways in laryngeal squamous cell carcinoma.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,11,(3)
MLA：
Yang, Yifan,et al."Array profiling identified dysregulated miRNAs and target genes and pathways in laryngeal squamous cell carcinoma".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 11..3(2018):1973-1983
