机构：[1]Department of Orthopaedics, The Second Hospital of Shanxi Medical University, Taiyuan, China.[2]Foot and Ankle Orthopaedic Surgery Center, Beijing Tongren Hospital, Beijing, China.首都医科大学附属北京同仁医院首都医科大学附属同仁医院[3]School of Community Health Science, Nevada Institute of Personalized Medicine, University of Nevada, Las Vegas, Nevada, USA.[4]Department of Orthopaedics, Affiliated Hospital of Guizhou Medical University, Guiyang, China.[5]Department of Orthopaedics, Warren Alpert Medical School of Brown University and Rhode Island Hospital, Providence, RI, USA.
Background: Malleolar fracture, which is present in 37-53% of human ankle osteoarthritis (OA), is the most common type of fracture in the ankle joint. In spite of this, no rat animal model has been developed for this type of injury to date. Here, we established a rat ankle post-traumatic OA (PTOA) model induced by malleolar fracture; this model will be useful in ankle OA research. Methods: Two-month-old male Sprague Dawley (SD) rats were randomized into 2 groups (n = 19 per group): 1) malleolus articular fracture, dislocation, and immediate reduction on the right joints and 2) malleolus articular fracture on the right ankle. The contralateral ankle joints were used as controls. The fracture and healing processes were confirmed and monitored by radiography. Changes in inflammation were monitored in vivo by fluorescence molecular tomography (FMT). Cartilage damage and changes in expression of OA-related genes were analyzed by histology, immunohistochemistry, Real-time quantitative PCR (qPCR) and enzyme-linked immunosorbent assay (ELISA) at 8 weeks post-surgery. Results: X-rays showed that all fractures were healed at 8 weeks post-surgery. A reproducible, mild to moderate degree of OA cartilage damage with reduced aggrecan was detected by histology in all animals in both groups but there was no significant difference between the two groups. Decreased Col-II and increased Col-X and MMP-13 levels were detected by qPCR, immunohistochemistry, ELISA and FMT from both groups cartilage. Conclusions: Malleolus articular fracture alone induces ankle OA with lesions on the central weight bearing area of the tibiotalar joint in rats. This model will provide a reproducible and useful tool for researchers to study ankle OA.
基金：
NIH/NIAMSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) [R01AR059142]; NSFCNational Natural Science Foundation of China (NSFC) [81171676, 31271033, 81572098, 81601949]; SXNSF [201308050, 20150313012-6, 201605D211024, 20161100006]; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [P30GM122732, P20GM104937] Funding Source: NIH RePORTER
第一作者机构：[1]Department of Orthopaedics, The Second Hospital of Shanxi Medical University, Taiyuan, China.
通讯作者：
通讯机构：[1]Department of Orthopaedics, The Second Hospital of Shanxi Medical University, Taiyuan, China.[5]Department of Orthopaedics, Warren Alpert Medical School of Brown University and Rhode Island Hospital, Providence, RI, USA.
推荐引用方式(GB/T 7714)：
Liang Dawei,Sun Jian,Wei Fangyuan,et al.Establishment of rat ankle post-traumatic osteoarthritis model induced by malleolus fracture[J].BMC MUSCULOSKELETAL DISORDERS.2017,18:doi:10.1186/s12891-017-1821-9.
APA：
Liang, Dawei,Sun, Jian,Wei, Fangyuan,Zhang, Jianzhong,Li, Pengcui...&Wei, Lei.(2017).Establishment of rat ankle post-traumatic osteoarthritis model induced by malleolus fracture.BMC MUSCULOSKELETAL DISORDERS,18,
MLA：
Liang, Dawei,et al."Establishment of rat ankle post-traumatic osteoarthritis model induced by malleolus fracture".BMC MUSCULOSKELETAL DISORDERS 18.(2017)
