Solute carrier family 7, membrane 11 (SLC7A11) or (xCT) is a component of the cysteine-glutamate transporter, which plays a critical role in glutathione homeostasis which is important to protect cells from oxidative stress. SLC7A11 is distributed in various tissues and participates in the occurrence of a number of diseases, particularly in the pathogenesis of malignant tumors, but its role in laryngeal cancer development has not yet been clearly defined. The objective of the present study was to investigate the role of SLC7A11 in laryngeal squamous cell carcinoma (LSCC). We conducted immunohistochemistry and RT-PCR to evaluate the protein and mRNA levels of SLC7A11 in LSCC and in control tissues, respectively. The knockdown experiments were conducted with SLC7A11 short hairpin RNA (shRNA) lentivirus, and the protein and mRNA levels of SLC7A11 were assessed by RT-PCR and western blotting. The functional study of SLC7A11 in vitro was conducted by MTT assay, and the effects on the cell cycle were detected using flow cytometry. Immunohistochemical results revealed that the expression levels of SLC7A11, Ki-67 and p53 in LSCC tissues were higher than those in laryngeal dysplasia tissues. The Spearman rank correlation analysis revealed that the expression of SLC7A11 was positively correlated with the expression of p53 and Ki-67. Cox regression analysis and Kaplan-Meier plots confirmed that the expression levels of SLC7A11 were a prognostic factor for overall survival (OS) rates and postoperative recurrence of LSCC. Moreover, the functional study of SLC7A11 in vitro revealed that knockdown of SLC7A11 using shRNA inhibited cell proliferation by inducing cell cycle arrest at the G1 phase. Immunohistochemical and RT-PCR results and knockdown experiments of SLC7A11 revealed that SLC7A11 was involved in the progression of LSCC, and may provide clinical information for the evaluation of OS rates and postoperative recurrence of LSCC. Collectively, these observations suggest that SLC7A11 may be a vital biomarker for the diagnosis and prognosis in human LSCC, and targeting SLC7A11 appears to be a potentially significant method for LSCC treatment.
基金：
National Natural Science Foundation Of ChinaNational Natural Science Foundation of China (NSFC) [81502493]; Capital Health Research and Development of Special Project [2014-2-2053]
第一作者机构：[1]Capital Med Univ, Dept Pathol, Beijing Key Lab Head & Neck Mol Diagnost Pathol, Beijing 100730, Peoples R China[4]Chengde Cent Hosp, Dept Otolaryngol, Chengde 067000, Hebei, Peoples R China
通讯作者：
通讯机构：[1]Capital Med Univ, Dept Pathol, Beijing Key Lab Head & Neck Mol Diagnost Pathol, Beijing 100730, Peoples R China[2]Capital Med Univ, Dept Otorhinolaryngol Head & Neck Surg, Beijing Tongren Hosp, 1 Dongjiaominxiang Rd, Beijing 100730, Peoples R China[3]Beijing Inst Otorhinolaryngol, Minist Educ, Key Lab Otorhinolaryngol Head & Neck Surg, Beijing 100005, Peoples R China[*1]Department of Pathology, Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing Tongren Hospital, Capital Medical University, 1 Dongjiaominxiang Road, Dongcheng, Beijing 100730, P.R. China[*2]Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 1 Dongjiaominxiang Road, Dongcheng, Beijing 100730, P.R. China
推荐引用方式(GB/T 7714)：
Ma Zhihong,Zhang Hong,Lian Meng,et al.SLC7A11, a component of cysteine/glutamate transporter, is a novel biomarker for the diagnosis and prognosis in laryngeal squamous cell carcinoma[J].ONCOLOGY REPORTS.2017,38(5):3019-3029.doi:10.3892/or.2017.5976.
APA：
Ma, Zhihong,Zhang, Hong,Lian, Meng,Yue, Changli,Dong, Gehong...&Liu, Hongang.(2017).SLC7A11, a component of cysteine/glutamate transporter, is a novel biomarker for the diagnosis and prognosis in laryngeal squamous cell carcinoma.ONCOLOGY REPORTS,38,(5)
MLA：
Ma, Zhihong,et al."SLC7A11, a component of cysteine/glutamate transporter, is a novel biomarker for the diagnosis and prognosis in laryngeal squamous cell carcinoma".ONCOLOGY REPORTS 38..5(2017):3019-3029
