The glucagon-like peptide 1 (GLP-1) analogue Exendin-4 (Ex-4) could perform anti-oxidative effects against oxidative damage both in vitro and in vivo. However, little was known about the direct protection of Exendin-4 on RPE cells against oxidative stress and its underlying mechanism. The present study examined the protective effects of Exendin-4 on RPE cells against H2O2 treatment in vitro and retina I/R injury in vivo. The results indicated that Ex-4 exerted protective effects under H2O2 condition on RPE cells and attenuated the oxidative stress-induced damage, as evidenced by the results of cellular viability, cytotoxicity, apoptosis and western blotting. However, the protective effects of Ex-4 were mainly abrogated by p-Akt inhibitor or p-STAT3 inhibitor. In conclusion, the Ex-4 was able to protect RPE cells from H2O2 induced oxidative damage in vitro and attenuate retinal injury from I/R in vivo, which was closely related to PI3k/Akt and p-STAT3 signaling.
第一作者机构:[1]Capital Med Univ, Beijing Chaoyang Hosp, Dept Ophthalmol, 5 Jingyuan Rd, Beijing, Peoples R China
通讯作者:
通讯机构:[1]Capital Med Univ, Beijing Chaoyang Hosp, Dept Ophthalmol, 5 Jingyuan Rd, Beijing, Peoples R China[*1]Department of Ophthalmology, Beijing Chaoyang Hospital, Capital Medical University, 5 Jingyuan Road, Shijingshan District, Beijing, People’s Republic of China
推荐引用方式(GB/T 7714):
Gao Min,Xiao Yang,Tian Lei.Exendin-4 protects retinal pigment epithelial cells against oxidative stress in vitro via PI3K/Akt-STAT3 activation[J].INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE.2017,10(6):9152-9161.
APA:
Gao, Min,Xiao, Yang&Tian, Lei.(2017).Exendin-4 protects retinal pigment epithelial cells against oxidative stress in vitro via PI3K/Akt-STAT3 activation.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,10,(6)
MLA:
Gao, Min,et al."Exendin-4 protects retinal pigment epithelial cells against oxidative stress in vitro via PI3K/Akt-STAT3 activation".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 10..6(2017):9152-9161