Aim: The goal of this study is to identify differentially methylated (DM) loci associated with long noncoding RNA (lncRNA)/mRNA expression in non-small-cell lung cancer (NSCLC). Materials & methods: Microarrays were used to interrogate genome-wide methylation and expression of lncRNA/mRNA in NSCLC. Results: We identified 113,644 DM loci between tumors and adjacent tissues. Among them, 26,310 DM loci were associated with 1685 differentially expressed genes, and 839 genes had significant correlations between methylation and expression, of which 26 hypermethylated loci in transcription start site 200 were correlated with low gene expression. We validated the correlations between methylation and expression in five genes (CDO1, C2orf40, SCARF1, ZFP106 and IFFO1) using pyrosequencing and quantitative polymerase chain reaction. We also found significant correlations between lncRNAs and mRNAs, and validated four of the correlations with quantitative polymerase chain reaction. Conclusion: Integrated analysis of genome-wide DNA methylation and lncRNA/mRNA expression allows us to identify new DM loci-correlated with gene expression in NSCLC.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81573231]; Natural Science Foundation of ShanghaiNatural Science Foundation of Shanghai [15ZR1424400]; Translational Medicine Foundation of SJTU medical college [15ZH1001]; EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENTUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) [R01HD084633] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Environmental Health Sciences (NIEHS) [K01ES025434] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [P20GM103457] Funding Source: NIH RePORTER
第一作者机构：[1]Shanghai Jiao Tong Univ, Sch Med, Hongqiao Int Inst Med, Shanghai Tongren Hosp, Shanghai 200025, Peoples R China[2]Shanghai Jiao Tong Univ, Sch Med, Fac Publ Hlth, Shanghai 200025, Peoples R China
通讯作者：
通讯机构：[1]Shanghai Jiao Tong Univ, Sch Med, Hongqiao Int Inst Med, Shanghai Tongren Hosp, Shanghai 200025, Peoples R China[2]Shanghai Jiao Tong Univ, Sch Med, Fac Publ Hlth, Shanghai 200025, Peoples R China
推荐引用方式(GB/T 7714)：
Feng Nannan,Wang Yu,Zheng Min,et al.Genome-wide analysis of DNA methylation and their associations with long noncoding RNA/ mRNA expression in non-small-cell lung cancer[J].EPIGENOMICS.2017,9(2):137-153.doi:10.2217/epi-2016-0120.
APA：
Feng, Nannan,Wang, Yu,Zheng, Min,Yu, Xiao,Lin, Hongyan...&Qian, Biyun.(2017).Genome-wide analysis of DNA methylation and their associations with long noncoding RNA/ mRNA expression in non-small-cell lung cancer.EPIGENOMICS,9,(2)
MLA：
Feng, Nannan,et al."Genome-wide analysis of DNA methylation and their associations with long noncoding RNA/ mRNA expression in non-small-cell lung cancer".EPIGENOMICS 9..2(2017):137-153
