Epigallocatechin-3-gallate (EGCG) has been shown to possess anti-inflammatory effects. Microcystin-LR (MC-LR) is a potent toxin and our past research suggested that it also mediated human umbilical vein endothelial cell (HUVEC) injury. The aim of this study was to investigate the effects of EGCG on MC-LR-induced oxidative stress and inflammatory responses in HUVECs. HUVECs were stimulated with MC-LR in the presence or absence of EGCG. MC-LR (40 AM) significantly increased cell death and decreased cell viability, migration, and tube formation, whereas EGCG (50 mu M) inhibited these effects. Furthermore, the results indicated that EGCG inhibited the production of reactive oxygen species (ROS), tumor necrosis factor alpha (TNF-alpha), and interleukin-6 (IL-6) in MC-LR-stimulated HUVECs. Compared with MC-LR, EGCG significantly increased superoxide dismutase (SOD) and glutathione (GSH) levels and decreased malondialdehyde (MDA) levels. Moreover, the analysis indicated that EGCG suppressed MC-LR-induced NF-kappa B activation. In conclusion, the effects of EGCG were associated with inhibition of the NF-kappa B signaling pathway, which resulted in decreased ROS and TNF-alpha, thereby attenuating MC-LR-mediated oxidative and inflammatory responses. (C) 2016 Elsevier Ltd. All rights reserved.
基金：
Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [81401870, 81400927]; Major Science and Technology Program for Water Pollution Control and Treatment, China [2014ZX07405002D]
第一作者机构：[1]Tongji Univ, Coll Environm Sci & Engn, Key Lab Yangtze River Water Environm, Shanghai, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Shi Jun,Deng Huiping,Pan Huichao,et al.Epigallocatechin-3-gallate attenuates microcystin-LR induced oxidative stress and inflammation in human umbilical vein endothelial cells[J].CHEMOSPHERE.2017,168:25-31.doi:10.1016/j.chemosphere.2016.10.037.
APA：
Shi, Jun,Deng, Huiping,Pan, Huichao,Xu, Yinjie&Zhang, Min.(2017).Epigallocatechin-3-gallate attenuates microcystin-LR induced oxidative stress and inflammation in human umbilical vein endothelial cells.CHEMOSPHERE,168,
MLA：
Shi, Jun,et al."Epigallocatechin-3-gallate attenuates microcystin-LR induced oxidative stress and inflammation in human umbilical vein endothelial cells".CHEMOSPHERE 168.(2017):25-31
环境科学与生态学