机构：[a]Department of Identification of Traditional Chinese Medicine,[b]Department of Pharmacology,The Second Military Medical University,[c]Hongqiao International Institute of Medicine, ShanghaiTongren Hospital and Faculty of Public Health, Shanghai Jiao Tong University School of Medicine,Shanghai,[d]Third Xiangya Hospital and the Institute of Vascular Disease and Translational Medicine,Central South University, Changsha, Hunan,[e]Department of Pharmacy, Shanghai Changzheng Hospital,The Second Military Medical University, Shanghai,[f]Department of Pharmacy, Shanghai East Hospital,Tongji University, Shanghai, China
Background/Aims: Prolonged fasting (PF) was shown to be of great potency to promote optimal health and reduce the risk of many chronic diseases. This study sought to determine the effect of PF on the endothelial progenitor cell (EPC)-mediated angiogenesis in the ischemic brain and cerebral ischemic injury in mice. Methods: Mice were subjected to PF or periodic PF after cerebral ischemia, and histological analysis and behavioral tests were performed. Mouse EPCs were isolated and examined, and the effects of EPC transplantation on cerebral ischemic injury were investigated in mice. Results: It was found that PF significantly increased the EP[ functions and angiogenesis in the ischemic brain, and attenuated the cerebral ischemic injury in mice that was previously subjected to cerebral ischemia. Periodic PF might reduce cortical atrophy and improve long-term neurobehavioral outcomes after cerebral ischemia in mice. The eNOS and MnSOD expression and intracellular NO level were increased, and TSP-2 expression and intracellular level were reduced in EPCs from PF-treated mice compared to control. In addition, transplanted EPCs might home into ischemic brain, and the EPCs from PF-treated mice had a stronger ability to promote angiogenesis in ischemic brain and reduce cerebral ischemic injury compared to the EPCs from control mice. The EPC-conditioned media from PF-treated mice exerted a stronger effect on cerebral ischemic injury reduction compared to that from control mice. Conclusion: Prolonged fasting promoted EPC-mediated ischemic angiogenesis and improved long-term stroke outcomes in mice. It is implied that prolonged fasting might potentially be an option to treat ischemic vascular diseases. (C) 2016 The Author(s) Published by S Karger AG, Basel
基金：
National Key Basic Research Program of China (973
Program; 2014CB542403 to A.F. Chen and H.H. Xie), the National Natural Science Foundation
of China (81370253 and 81170115 to H.H. Xie; 81603097 to X.H. Dong), the National Science
Foundation of China Key Research Project (81130004 to A.F. Chen).
第一作者机构：[a]Department of Identification of Traditional Chinese Medicine,
共同第一作者：
通讯作者：
通讯机构：[a]Department of Identification of Traditional Chinese Medicine,[b]Department of Pharmacology,The Second Military Medical University,[c]Hongqiao International Institute of Medicine, ShanghaiTongren Hospital and Faculty of Public Health, Shanghai Jiao Tong University School of Medicine,Shanghai,[d]Third Xiangya Hospital and the Institute of Vascular Disease and Translational Medicine,Central South University, Changsha, Hunan,[*1]Hongqiao Int Institute of Medicine and Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025 (China).Dept Pharmacol and Dept Identification of Traditional Chinese Medicine, The Second Military Medical University, 325 Guo He Road, Shanghai 200433 (China)
推荐引用方式(GB/T 7714)：
Xin Bao,Liu Chun-Long,Yang Hong,et al.Prolonged Fasting Improves Endothelial Progenitor Cell-Mediated Ischemic Angiogenesis in Mice[J].CELLULAR PHYSIOLOGY AND BIOCHEMISTRY.2016,40(3-4):693-706.doi:10.1159/000452581.
APA：
Xin, Bao,Liu, Chun-Long,Yang, Hong,Peng, Cheng,Dong, Xiao-Hui...&Xie, He-Hui.(2016).Prolonged Fasting Improves Endothelial Progenitor Cell-Mediated Ischemic Angiogenesis in Mice.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,40,(3-4)
MLA：
Xin, Bao,et al."Prolonged Fasting Improves Endothelial Progenitor Cell-Mediated Ischemic Angiogenesis in Mice".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 40..3-4(2016):693-706
