机构:[1]Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730临床科室麻醉科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]Department of Neurobiology, Capital Medical University,Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069[3]Department of Anesthesiology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038[4]Department of Anesthesiology, Beijing Stomatological Hospital, Capital Medical University[5]Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China首都医科大学附属北京友谊医院
It has been reported that morphine pretreatment (MP) can exert neuroprotective effects, and that protein kinase C (PKC) participates in the initiation and development of ischemic/hypoxic preconditioning in the brain. However, it remains unknown whether PKC is involved in MP-induced neuroprotection. The aim of the present study, which included in vivo and in vitro experiments, was to determine whether the conventional gamma isoform of PKC (cPKC gamma) was involved in the protective effects of MP against cerebral ischemic injury. The present study included an in vivo experiment using a mouse model of middle cerebral artery occlusion and an in vitro experiment using neuroblastoma N2a cells with oxygen-glucose deprivation (OGD). Furthermore, a cPKC gamma antagonist, Go6983, was used to determine the involvement of cPKC gamma in the protective effects of MP against cerebral ischemic injury. In the in vivo experiment, neurological deficits, ischemic infarct volume, neural cell damage, apoptosis and caspase-3 activation were evaluated. In the in vitro experiment, flow cytometry was used to determine the activation of caspase-3 in N2a cells with OGD. It was found that MP protected against cerebral ischemic injury. However, intracerebroventricular injection of the cPKC gamma antagonist before MP attenuated the neuroprotective effect of MP and increased the activation of cleaved caspase-3. These findings suggested that MP may provide protection against cerebral ischemic injury via a cPKC gamma-mediated anti-apoptosis pathway.
基金:
The present study was supported by the National Science
Foundation of China (grant nos. 81671044, 81970344,
314711475 and 31671205).
第一作者机构:[1]Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730
通讯作者:
通讯机构:[1]Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730[5]Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China[*1]Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, 1 Dongjiaominxiang, Dong‑Cheng, Beijing 100730, P.R. China[*2]Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, 95 Yong‑An Road, Xi‑Cheng, Beijing 100050, P.R. China
推荐引用方式(GB/T 7714):
XIAO‑YAN ZHAO,JUN‑FA LI,TIAN‑ZUO LI,et al.Morphine pretreatment protects against cerebral ischemic injury via a cPKCγ-mediated anti-apoptosis pathway[J].EXPERIMENTAL AND THERAPEUTIC MEDICINE.2021,22(3):doi:10.3892/etm.2021.10448.
APA:
XIAO‑YAN ZHAO,JUN‑FA LI,TIAN‑ZUO LI,CHU‑XIONG PAN,FU‑SHAN XUE&GU‑YAN WANG.(2021).Morphine pretreatment protects against cerebral ischemic injury via a cPKCγ-mediated anti-apoptosis pathway.EXPERIMENTAL AND THERAPEUTIC MEDICINE,22,(3)
MLA:
XIAO‑YAN ZHAO,et al."Morphine pretreatment protects against cerebral ischemic injury via a cPKCγ-mediated anti-apoptosis pathway".EXPERIMENTAL AND THERAPEUTIC MEDICINE 22..3(2021)