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Morphine pretreatment protects against cerebral ischemic injury via a cPKCγ-mediated anti-apoptosis pathway

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机构: [1]Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730 [2]Department of Neurobiology, Capital Medical University,Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069 [3]Department of Anesthesiology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038 [4]Department of Anesthesiology, Beijing Stomatological Hospital, Capital Medical University [5]Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China
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关键词: middle cerebral artery occlusion morphine mice neuroprotection protein kinase C

摘要:
It has been reported that morphine pretreatment (MP) can exert neuroprotective effects, and that protein kinase C (PKC) participates in the initiation and development of ischemic/hypoxic preconditioning in the brain. However, it remains unknown whether PKC is involved in MP-induced neuroprotection. The aim of the present study, which included in vivo and in vitro experiments, was to determine whether the conventional gamma isoform of PKC (cPKC gamma) was involved in the protective effects of MP against cerebral ischemic injury. The present study included an in vivo experiment using a mouse model of middle cerebral artery occlusion and an in vitro experiment using neuroblastoma N2a cells with oxygen-glucose deprivation (OGD). Furthermore, a cPKC gamma antagonist, Go6983, was used to determine the involvement of cPKC gamma in the protective effects of MP against cerebral ischemic injury. In the in vivo experiment, neurological deficits, ischemic infarct volume, neural cell damage, apoptosis and caspase-3 activation were evaluated. In the in vitro experiment, flow cytometry was used to determine the activation of caspase-3 in N2a cells with OGD. It was found that MP protected against cerebral ischemic injury. However, intracerebroventricular injection of the cPKC gamma antagonist before MP attenuated the neuroprotective effect of MP and increased the activation of cleaved caspase-3. These findings suggested that MP may provide protection against cerebral ischemic injury via a cPKC gamma-mediated anti-apoptosis pathway.

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出版当年[2020]版:
大类 | 4 区 医学
小类 | 4 区 医学:研究与实验
最新[2023]版:
大类 | 4 区 医学
小类 | 4 区 医学:研究与实验
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出版当年[2019]版:
Q4 MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版:
Q3 MEDICINE, RESEARCH & EXPERIMENTAL

影响因子: 最新[2023版] 最新五年平均 出版当年[2019版] 出版当年五年平均 出版前一年[2018版] 出版后一年[2020版]

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第一作者机构: [1]Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730
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通讯机构: [1]Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730 [5]Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China [*1]Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, 1 Dongjiaominxiang, Dong‑Cheng, Beijing 100730, P.R. China [*2]Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, 95 Yong‑An Road, Xi‑Cheng, Beijing 100050, P.R. China
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