机构：[1]Department of Surgery, Memorial Sloan–Kettering Cancer Center, New York, New York[2]Department of Otorhinolaryngology, Mayo Clinic, Rochester, Minnesota[3]Department of Otolaryngology – Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology – Head and Neck Surgery, Ministry of Education, Beijing, China临床科室耳鼻咽喉-头颈外科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[4]Genelux Corporation, San Diego Science Center, San Diego, California[5]Department of Radiation Oncology, Rebecca & John Moores Comprehensive Cancer Center, University of California, San Diego, California[6]Rudolf Virchow Center for Experimental Biomedicine, Institute for Biochemistry and Institute for Molecular Infection Biology, University of Wurzburg, Am Hubland, Wurzburg, Germany[7]Protein Polymer Technologies Inc., San Diego, California[8]Department of Surgery, City of Hope, Duarte, California.
BackgroundOncolytic viral efficacy may be limited by the penetration of the virus into tumors. This may be enhanced by intraoperative application of virus immediately after surgical resection. MethodsOncolytic vaccinia virus GLV-1h68 was delivered in silk-elastin-like protein polymer (SELP) in vitro and in vivo in anaplastic thyroid carcinoma cell line 8505c in nude mice. ResultsGLV-1h68 in SELP infected and lysed anaplastic thyroid cancer cells in vitro equally as effectively as in phosphate-buffered saline (PBS), and at 1 week retains a thousand fold greater infectious plaque-forming units. In surgical resection models of residual tumor, GLV-1h68 in SELP improves tumor control and shows increased viral -galactosidase expression as compared to PBS. ConclusionThe use of SELP matrix for intraoperative oncolytic viral delivery protects infectious viral particles from degradation, facilitates sustained viral delivery and transgene expression, and improves tumor control. Such optimization of methods of oncolytic viral delivery may enhance therapeutic outcomes. (c) 2014 Wiley Periodicals, Inc. Head Neck 38: 237-246, 2016
基金：
National Cancer InstituteUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Cancer Institute (NCI) [T32CA009685]; National Institute for Dental and Craniofacial ResearchUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Dental & Craniofacial Research (NIDCR) [R21DE019015]; Flight Attendant Medical Research Institute; NATIONAL CANCER INSTITUTEUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Cancer Institute (NCI) [P30CA008748, T32CA009685] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Dental & Craniofacial Research (NIDCR) [R21DE019015] Funding Source: NIH RePORTER
第一作者机构：[1]Department of Surgery, Memorial Sloan–Kettering Cancer Center, New York, New York[2]Department of Otorhinolaryngology, Mayo Clinic, Rochester, Minnesota[*1]Department of Otorhinolaryngology, Mayo Clinic, 200 1st ST SW, Rochester, MN 55902
通讯作者：
通讯机构：[1]Department of Surgery, Memorial Sloan–Kettering Cancer Center, New York, New York[2]Department of Otorhinolaryngology, Mayo Clinic, Rochester, Minnesota[*1]Department of Otorhinolaryngology, Mayo Clinic, 200 1st ST SW, Rochester, MN 55902[*2]Head and Neck Service, C-1069, Department of Surgery, Memorial Sloan–Kettering Cancer Center, New York, NY 10021.
推荐引用方式(GB/T 7714)：
Price Daniel L.,Li Pingdong,Chen Chun-Hao,et al.Silk-elastin-like protein polymer matrix for intraoperative delivery of an oncolytic vaccinia virus[J].HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK.2016,38(2):237-246.doi:10.1002/hed.23877.
APA：
Price, Daniel L.,Li, Pingdong,Chen, Chun-Hao,Wong, Danni,Yu, Zhenkun...&Wong, Richard J..(2016).Silk-elastin-like protein polymer matrix for intraoperative delivery of an oncolytic vaccinia virus.HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK,38,(2)
MLA：
Price, Daniel L.,et al."Silk-elastin-like protein polymer matrix for intraoperative delivery of an oncolytic vaccinia virus".HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK 38..2(2016):237-246
