机构：[1]Department of Interventional Radiology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai[2]Department of Interventional Radiology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, PR China
Background/Aims: Emerging evidence indicates that microRNA (miR)-340 is downregulated in various human cancers, suggesting that it acts as a tumor suppressor. The aim of the present study was to evaluate the expression and role of miR-340 in human esophageal squamous cell carcinoma (ESCC). Methods: The expression of miR-340 was examined in 64 paired ESCC and adjacent non-tumor tissues by quantitative real time PCR. The effects of miR-340 on ESCC cell proliferation and metastasis were examined by MTT and Matrigel invasion assays. Tumor growth was assessed by subcutaneous inoculation of cells into BALB/c nude mice. Targets of miR-340 were identified by bioinformatics and verified by luciferase reporter assays, quantitative real-time PCR, and western blotting. Results: MiR-340 was significantly downregulated in ESCC tumor tissues compared to adjacent non-tumor tissues and in ESCC cell lines compared to esophageal endothelial cells. Overexpression of miR-340 inhibited ESCC cell growth, colony formation, and invasion, and tumor growth in a xenograft mouse model. PSAT1 was identified as a direct target of miR-340 and its ectopic expression partially reversed the miR-340 mediated inhibition of viability, invasion and EMT in ESCC cells. The expression of miR-340 was negatively correlated with that of PSAT1 in human ESCC samples. Conclusion: MiR-340 functions as a tumor suppressor by modulating the expression of PSAT1 and may contribute to the progression and invasiveness of ESCC. Copyright,(C) 2015 S. Karger AG, Basel
第一作者机构：[1]Department of Interventional Radiology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai
共同第一作者：
通讯作者：
通讯机构：[1]Department of Interventional Radiology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai[*1]Department of Interventional Radiology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, No.786,Yuyuan Road, Shanghai 200050 (China) and Department of Oncology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200050, (China)
推荐引用方式(GB/T 7714)：
Yan Shuo,Jiang Haosheng,Fang Shiming,et al.MicroRNA-340 Inhibits Esophageal Cancer Cell Growth and Invasion by Targeting Phosphoserine Aminotransferase 1[J].CELLULAR PHYSIOLOGY AND BIOCHEMISTRY.2015,37(1):375-386.doi:10.1159/000430361.
APA：
Yan, Shuo,Jiang, Haosheng,Fang, Shiming,Yin, Fang,Wang, Zhenlei...&Mao, Aiwu.(2015).MicroRNA-340 Inhibits Esophageal Cancer Cell Growth and Invasion by Targeting Phosphoserine Aminotransferase 1.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,37,(1)
MLA：
Yan, Shuo,et al."MicroRNA-340 Inhibits Esophageal Cancer Cell Growth and Invasion by Targeting Phosphoserine Aminotransferase 1".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 37..1(2015):375-386
